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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Nat Methods. 2016 Oct 24;13(12):1043–1049. doi: 10.1038/nmeth.4042

Complex transcriptional modulation with orthogonal and inducible dCas9 regulators

Yuchen Gao 1,2,9, Xin Xiong 3,4,9, Spencer Wong 3, Emeric J Charles 1, Wendell A Lim 3,4,5,6, Lei S Qi 1,7,8
PMCID: PMC5436902  NIHMSID: NIHMS858250  PMID: 27776111

Abstract

The ability to dynamically manipulate the transcriptome is important for studying how gene networks direct cellular functions and how network perturbations cause disease. Nuclease-dead CRISPR–dCas9 transcriptional regulators, while offering an approach for controlling individual gene expression, remain incapable of dynamically coordinating complex transcriptional events. Here, we describe a flexible dCas9-based platform for chemical-inducible complex gene regulation. From a screen of chemical- and light-inducible dimerization systems, we identified two potent chemical inducers that mediate efficient gene activation and repression in mammalian cells. We combined these inducers with orthogonal dCas9 regulators to independently control expression of different genes within the same cell. Using this platform, we further devised AND, OR, NAND, and NOR dCas9 logic operators and a diametric regulator that activates gene expression with one inducer and represses with another. This work provides a robust CRISPR—dCas9-based platform for enacting complex transcription programs that is suitable for large-scale transcriptome engineering.


The cellular transcriptome is a convergence point that integrates myriad signals to execute biological processes. During development, the transcriptional programs of stem cells coordinate dynamic changes to the mRNA abundance of key genes, driving differentiation into various cell types. Transcriptome disruption is a hallmark of disease. Genome-wide association studies demonstrate that the majority (>93%) of cellular trait- and disease-associated variants exist within noncoding DNA, highlighting the essential role of transcriptional regulation in these processes1,2. While techniques such as DNA microarrays and RNA-seq enable reading of the cellular transcriptome3,4, researchers must be able to manipulate transcription in a multiplexed and dynamic fashion in order to establish causal relationships between gene networks and cellular function. Conventional small-molecule-inducible transgene expression systems based on doxycycline or ponasterone A allow manipulation of individual genes5,6, yet they lack the flexibility and scalability required for simultaneous multigene modulation. Repurposing genome engineering technologies may offer a promising solution for flexible transcriptome engineering of complex gene networks.

The bacterial CRISPR—Cas9 system is a versatile RNA-guided platform for genome engineering in cell culture or in vivo systems7. Nuclease Cas9 protein, when paired with a single guide RNA (sgRNA), can be used to precisely edit genomic DNA811. Beyond editing, nuclease-dead Streptococcus pyogenes Cas9 (D10A and H840A), termed dCas9, can be combined with transcriptional activation or repression effectors for sequence-specific constitutive activation or gene repression in mammalian systems1217. Furthermore, proof-of-concept work has shown that dCas9 can be fused to light- and chemical-inducible domains to conditionally activate gene expression using extrinsic signals1820. While these inducible dCas9 transcriptional regulators enable regulation of individual genes in isolation, engineering dCas9 regulators that simultaneously utilize multiple inducible systems remains an unexplored area of research. If such inducible systems can act without crosstalk in the same cell, then they can be used in parallel on different dCas9s to dynamically regulate independent gene targets or in combination on a single dCas9 to regulate one target using multiple signals. Given the flexibility and ease of use of CRISPR—Cas9, such advances could provide an approach for the complex regulation of gene expression and for the study of sophisticated gene networks. In addition, the resulting regulators would expand the toolkit of functional parts available for building dCas9-based synthetic gene circuits in mammalian cells21.

Here, we present a dCas9-based platform for engineering inducible and orthogonal transcriptional regulators to produce complex transcriptional programs. We systematically explore how dCas9 can be coupled with chemical- and light-inducible domains to dynamically regulate gene activation and repression. These technologies enable the manipulation of multiple gene targets in distinct regulatory patterns and can be utilized for applications requiring reversible and highly specific control over gene transcription such as developmental and disease modeling.

RESULTS

Screening chemical- or light-inducible dCas9s for efficient gene activation

We designed an inducible dCas9-based transcriptional regulation system in which dCas9 and a transcriptional effector are fused to complementary pairs of heterodimerization domains and only assemble in the presence of an extrinsic inducer. To test this design, we systematically fused S. pyogenes (Sp) dCas9 and a VPR (VP64-p65-Rta) activator22 to six previously reported chemical-and light-inducible heterodimerization domains cloned onto a dual-promoter PiggyBac vector (Supplementary Fig. 1a). These heterodimerization domains consisted of abscisic acid (ABA)-inducible ABI–PYL1 (ref. 23), gibberellin (GA)-inducible GID1–GAI24, rapamycin-inducible FKBP–FRB25, phytochrome-based red light-inducible PHYB–PIF26, cryptochrome-based blue light-inducible CRY2PHR–CIBN27, and light oxygen voltage-based blue-light-inducible FKF1–GI28.

We assessed the activation efficiency of each inducible dCas9 in HEK293T cells by targeting a Tet-On promoter (pTRE3G)–EGFP reporter (Supplementary Fig. 1b). The most potent systems were the ABA and GA heterodimerization systems, both derived from plant hormone signaling pathways23,24. Upon induction, the ABA- and GA-dimerized VPR–Sp-dCas9-activated EGFP protein expression 165- and 94-fold, respectively (Fig. 1a,b), efficiencies comparable to that of a VPR–Sp dCas9 direct fusion protein (Supplementary Fig. 2a). Critically, these inducible dCas9s showed no leaky activation when expressed without inducer. Rather, we observed a minor decrease (10–30%) in EGFP relative to reporter-only cells (Fig. 1a,b), an effect likely due to steric interference with native transcription factors from dCas9 occupancy on the promoter12. Furthermore, addition of inducers to reporter cells without dCas9 elicited no increase in EGFP relative to uninduced cells, excluding nonspecific activation. In contrast, the three light-inducible systems and the rapamycin system showed modest EGFP activation (1.3- to 5.6-fold) upon induction (Supplementary Fig. 1c–f). Previous studies found that light-inducible dimerizing domains exhibit low light sensitivity29 and highly context-dependent structural folding30, which may explain the weak response. In addition, we tested exchanging dimerization domains between the dCas9 and effector domains and found that many inverted dCas9 activators showed poor activity (Supplementary Fig. 3). These results suggest that dimerization of the inducible domains may be sensitive to their spatial orientation relative to dCas9.

Figure 1.

Figure 1

A modular dCas9 platform for inducible gene activation and repression. (a,b) Fluorescence quantification after 48 h induction for HEK293T pTRE3G–EGFP cells transfected with Sp sgTRE3G and (a) ABA-inducible or (b) GA-inducible VPR–Sp dCas9. (c,d) Fluorescence quantification after 5 d induction for HEK293T pSV40–EGFP cells transfected with Sp sgSV40 and (c) ABA-inducible or (d) GA-inducible Sp KRAB–dCas9. Inverse fold-change repression is indicated in magenta. (e,f) Fluorescence quantification after 48 h for HEK293T pTRE3G–EGFP cells transfected with Sa sgTRE3G and (e) ABA-inducible or (f) GA-inducible VPR-Sa dCas9. (g) Immunofluorescence quantification of CXCR4 after 48 h induction in HEK293T cells transfected with Sp sgCXCR4 and ABA-inducible dCas9 fused to a VPR, SunTag–VP64, or SunTag–VPR activation domain. For the no-sgRNA controls in (g), the data represent two independent transfections performed in technical replicates (n = 4); for other conditions in (g) and all other experiments, the data represent four independent transfections performed in technical replicates (n = 8). Mean fluorescence intensities are presented as arbitrary units (a.u.). P values from Games–Howell post hoc tests following Welch’s ANOVA are provided in supplementary Table 1.

Implementing inducible repression of gene expression

To expand the inducible system to gene repression, we replaced the effector fused to the ABA- or GA-inducible dimerization domains with the Krüppel-associated box (KRAB) repression domain31.

We assessed inducible repression in HEK293T cells by targeting a constitutive SV40 promoter (pSV40)–EGFP reporter (Supplementary Fig. 4a). The ABA- and GA-inducible systems repressed EGFP 5.6- and 3.2-fold, respectively (Fig. 1c,d), with efficiencies comparable to that of a KRAB-Sp dCas9 direct fusion protein (Supplementary Fig. 2b). Binding of dCas9 alone to pSV40 in uninduced cells showed no or only slight reduction of EGFP signals relative to reporter-only cells for either inducible system (Fig. 1c,d). These results suggest that promoter targeting provides a viable strategy for inducible repression by maximizing the induced repressive effect from KRAB recruitment while minimizing the basal repressive effect of steric hindrance from promoter-bound dCas9 alone.

Staphylococcus aureus inducible dcas9 transcriptional regulators

Recent work showed that a Cas9 protein derived from S. aureus is capable of gene editing32 and transcriptional activation33,34 in mammalian cells. We tested whether our inducible fusion protein design would transfer to nuclease-dead S. aureus Cas9 (Sa dCas9). Both ABA- and GA-inducible activators using Sa dCas9 increased EGFP expression in HEK293T pTRE3G-EGFP cells (Fig. 1e,f) and achieved similar levels of activation as direct fusion VPR–Sa dCas9 (Supplementary Fig. 2c). However, Sa dCas9 activated EGFP to 20-30% the levels of Sp dCas9 overall, possibly because of differences in sgRNA targeting sequence efficiencies, the lack of a functionally optimized Sa sgRNA scaffold35, or intrinsic differences between dCas9 DNA-binding affinities. Nonetheless, retention of gene activation indicates that our fusion design can transfer to multiple Cas9 orthologs.

Inducible activation of endogenous genes

We further validated the activity of ABA-inducible Sp dCas9 on an endogenous gene. Here, we introduced individual dCas9 constructs into HEK293T cells along with an sgRNA targeting the CXCR4 gene promoter. We compared activation using the VPR, SunTag–VP6436, or combined SunTag–VPR effectors (Fig. 1g). For these effectors, ABA induction increased CXCR4 protein levels 6.4- to 8.9-fold relative to the levels of uninduced cells. The inability of the combined SunTag–VPR effector to further increase activation efficiency is corroborated by a recent comparative study of multiple Cas9 activator systems37.

Reversibility and dose responsiveness of ABA- and GA-inducible regulation

We subsequently performed detailed dynamic characterizations of the ABA and GA systems. For activation, we monitored EGFP levels over 7 d in HEK293T pTRE3G–EGFP cells stably expressing ABA-or GA-inducible Sp VPR–dCas9 as well as in cells expressing the Sa counterparts. In all systems tested, cells under continuous inducer treatment over 7 d maintained high levels of EGFP (Fig. 2a,b). Removal of the inducer after 2 d triggered a progressive decrease in EGFP levels to baseline, demonstrating reversibility of activation. Furthermore, readdition of inducer in these cells activated EGFP as strongly and rapidly as the original treatment. GA-inducible systems exhibited slower reversal kinetics upon inducer removal (Fig. 2b), possibly on account of residual GA in the media or slow dissociation of GA-dimerized proteins. The dissociation constant of GA has been estimated to be 10 to 100 times lower than that of ABA38,39. We also quantified dose responses for the four activation systems by measuring EGFP after inducing with varying concentrations of ABA or GA (Fig. 2c,d). We observed wide linear ranges of ABA and GA responsiveness, implying that dosage can modulate activation strength. At the highest tested dose of GA (100 μM), EGFP levels declined (Fig. 2d), possibly owing to a cell acidification effect previously described24.

Figure 2.

Figure 2

Characterization of the dynamics and dose response of ABA-and GA-inducible gene activation. (a,b) 7-d timecourse for clonal HEK293T pTRE3G-EGFP cells stably expressing Sp or Sa sgTRE3G and (a) ABA-inducible or (b) GA-inducible VPR-Sp or Sa dCas9. Cells were continuously induced with 100 μM ABA or 10 μM GA for 7 d (ON7); induced for 2 d then cultured without inducer for 5 d (ON2 OFF5); or induced for 2 d, cultured without inducer for 3 d, then reinduced for 2 d (ON2 OFF3 ON2). The data are displayed as individual data points for two independent platings of a stable cell line (n = 2). (c,d) Dose response after 48 h induction for HEK293T pTRE3G–EGFP cells transfected with Sp or Sa sgTRE3G and (c) ABA-inducible or (d) GA-inducible VPR–Sp or Sa dCas9. The shaded regions indicate the approximate linear dose–response ranges. The data are displayed as mean ± s.d. for four independent transfections (n = 4).

We additionally characterized the dynamics of the ABA-inducible KRAB–Sp dCas9 repression system. Similar to activation, ABA-induced repression was maintained over 7 d under continuous treatment (Supplementary Fig. 4b). Upon removal of ABA, EGFP continued to decrease for 24 h before recovering. We suspect that this delay was caused by the lingering effects of epigenetic modifications initiated by KRAB40. ABA-inducible repression also demonstrated dose-responsive behavior, as the repressive effect increased at higher doses (Supplementary Fig. 4c). Unlike activation, repression was observed at low doses, which may indicate that various transcriptional effectors require different effective concentrations at the promoter to achieve regulation.

Orthogonal gene regulation by inducible orthogonal dcas9 regulators

The addition of a second, independently controlled dCas9 transcriptional regulator increases the flexibility and scope of dCas9 tools to allow simultaneous regulation of orthogonal genes. We evaluated whether inducible Sa dCas9 could be used alongside Sp dCas9 in the same cell without crosstalk. To achieve complete orthogonality, two criteria must be met: (i) the two inducers must not cross-activate or interfere with each other; and (ii) each of the dCas9s must not interact with the sgRNAs of the other dCas9 (Supplementary Fig. 5a). To address the first criterion, we demonstrated that ABA could not induce heterodimerization of GA-inducible Sp dCas9 and did not interfere with the ability of GA to dimerize GA-inducible Sp dCas9 (Supplementary Fig. 5b). Conversely, GA did not cross-induce or block dimerization of ABA-inducible Sp dCas9. Next, using different combinations of dCas9 activators and sgRNAs, we showed that gene activation only occurred when Sp dCas9 was expressed with Sp sgRNA or Sa dCas9 was expressed with Sa sgRNA, suggesting the absence of crosstalk between dCas9 orthologs and sgRNAs (Supplementary Fig. 5c,d).

We then used the constitutive, direct fusion forms of KRAB–Sp dCas9 and VPR–Sa dCas9 to demonstrate simultaneous regulation of two genes, one activated and one repressed. We expressed Sp and/or Sa dCas9 constructs in a HEK293T pSV40–EGFP pTRE3G–mCherry dual reporter cell line along with Sp sgSV40 and/or Sa sgTRE3G (Fig. 3a). Cells only expressing KRAB–Sp dCas9 and Sp sgSV40 repressed EGFP, while cells only expressing VPR–Sa dCas9 and Sa sgTRE3G activated mCherry. Cells coexpressing both sets of dCas9s and sgRNAs concurrently repressed EGFP and activated mCherry.

Figure 3.

Figure 3

Orthogonal gene regulation by independently inducible dCas9s. (a,b) Fluorescence quantifications for HEK293T pSV40-EGFP pTRE3G-mCherry dual reporter cells transfected with (a) direct fusion KRAB-Sp dCas9 and/or VPR-Sa dCas9 plus their respective sgRNAs or (b) ABA-inducible KRAB–Sp dCas9, GA-inducible VPR–Sa dCas9, and their respective sgRNAs. EGFP fluorescence is displayed for quantifications performed after 5 d induction, and mCherry fluorescence is displayed for quantifications performed after 48 h induction. Fold-change activation is indicated in black, while inverse fold-change repression is indicated in magenta. The data represent four independent transfections performed in technical replicates (n = 8). Dots represent individual data points. The different colors represent different treatment conditions: gray, no inducer; yellow, ABA; green, GA; blue, both ABA and GA. (c,d) Immunofluorescence quantifications for CXCR4 and CD95 after 48 h induction for HEK293T cells transiently transfected with (c) direct fusion KRAB–Sp dCas9, VPR–Sa dCas9, and sgRNAs or (d) GA-inducible VPR–Sp dCas9, ABA-inducible VPR–Sa dCas9, and sgRNAs and stained with APC-conjugated CXCR4 and PE-conjugated CD95 antibodies. Transfected sgRNAs consisted of three targeting sgRNAs (Sp sgCD95-1 to 3 or Sa sgCXCR4-1 to 3) per gene of interest or an equimolar amount of nontargeting sgRNA (Sp/Sa sgTRE3G). The data represent four independent transfections (n = 4). P values from Games-Howell post hoc tests following Welch’s ANOVA are provided in supplementary Table 1.

Finally, we cointroduced ABA-inducible KRAB–Sp dCas9 and GA-inducible VPR–Sa dCas9 into dual reporter cells along with Sp sgSV40 and Sa sgTRE3G (Fig. 3b). We observed that cells only induced with ABA repressed EGFP, and cells only induced with GA activated mCherry. Cells given both inducers simultaneously activated mCherry and repressed EGFP. Importantly, 2D flow cytometry plots show that under simultaneous ABA and GA induction, the subpopulation of cells with increased mCherry also exhibited decreased EGFP, confirming that the same cells are responding to both inducers (Supplementary Fig. 6a).

Orthogonal and inducible endogenous gene activation

We further showed that the dCas9 orthologs can independently regulate expression of discrete endogenous gene targets. In HEK293T cells, direct fusion VPR–Sp dCas9 activated CD95 expression, and VPR–Sa dCas9 concurrently activated CXCR4 expression when coexpressed with their respective sgRNAs (Fig. 3c). When GA-inducible VPR–Sp dCas9 and ABA-inducible VPR–Sa dCas9 were coexpressed instead, GA and ABA could independently trigger activation of CD95 and CXCR4 expression, respectively (Fig. 3d). 2D flow cytometry plots show that under induction by both GA and ABA, individual cells simultaneously expressed higher levels of CD95 and CXCR4 (Supplementary Fig. 6b). Thus, these results demonstrate that two inducible dCas9 transcriptional regulators can act in one cell in an orthogonal fashion.

Coupling multiple chemical-inducible domains for Boolean logic operations

We then looked to expand the vertical flexibility of dCas9 regulators by adding additional layers of inducible control to a single dCas9. Given the robustness of the ABA- and GA-inducible systems, we combined them to generate transcriptional Boolean logic gates. We first created an OR gate by fusing the GA-inducible GAI domain onto the N terminus and the ABA-inducible ABI domain onto the C terminus of Sp dCas9 (Fig. 4a). When coexpressing this dCas9 protein with both GID1–VPR and PYL1–VPR, HEK293T pTRE3G–EGFP cells showed strong EGFP activation (76- to 139-fold) upon induction with ABA alone, GA alone, or both ABA and GA (Fig. 4a).

Figure 4.

Figure 4

A multi-input CRISPR system for complex regulation of gene expression. (a,b) Fluorescence quantifications after 48 h induction for HEK293T pTRE3G–EGFP cells transiently transfected with Sp sgTRE3G and (a) the OR gate or (b) the AND gate VPR–Sp dCas9 construct. The dotted line represents the mean EGFP fluorescence in uninduced cells. The data represent four independent transfections performed in technical replicates (n = 8). (c) Schematic representation of inducibly recruiting opposing transcriptional effectors to a single dCas9. (d) Fluorescence quantifications after 5 d induction for HEK293T pSV40–EGFP cells transfected with the diametric Sp dCas9 construct and Sp sgSV40. Fold-change activation is indicated in black, while inverse fold-change repression is indicated in magenta. The data represent two independent transfections performed in technical replicates (n = 4). (e) 7-d inducer switch timecourse of HEK293T pSV40–EGFP cells stably expressing the diametric Sp dCas9 construct and Sp sgSV40. Cells were induced 2 d with 10 μM GA then replaced with 100 μM ABA for 5 d. Alternatively, cells were induced 5 d with 100 μM ABA then replaced with 10 μM GA for 2 d. The dotted line represents the mean EGFP fluorescence in uninduced cells. The data represent individual data points for two independent platings of a stable cell line (n = 2). P values from Games–Howell post hoc tests following Welch’s ANOVA are provided in supplementary Table 1.

We next sought to generate an AND gate by serially linking two heterodimerization domains (Fig. 4b). The GA-inducible GAI domain was fused to dCas9, the ABA-inducible PYL1 domain to VPR, and the ABI domain to GID1. While cells coexpressing these constructs showed no increase in EGFP when treated with either inducer alone, simultaneous treatment with both inducers produced a 49-fold increase in EGFP (Fig. 4b). We also exchanged the positions of the ABA-inducible and GA-inducible domains and found retention of AND gate function (Supplementary Fig. 7a). Furthermore, we used analogous designs to successfully generate NOR and NAND gates by replacing VPR with KRAB, though NAND showed modest repression (Supplementary Fig. 7b,c). Our results demonstrate an expandable, modular framework for integrating two or more signals into a single dCas9 regulator for logic-based control of gene expression.

Implementing a dual-input inducible dcas9 for diametric gene regulation

Diametric upregulation and downregulation of genes provides a flexible approach for functional interrogation by extending the dynamic range of expression. We created a diametric dCas9 system induced by different inputs to alternatively recruit opposing transcriptional effectors that activate or repress gene expression. This system utilizes the dCas9 from the OR gate coexpressed with GID1–VPR and PYL1–KRAB (Fig. 4c). In pSV40–EGFP cells, ABA-induced recruitment of KRAB resulted in 2.7-fold EGFP repression, while GA-induced recruitment of VPR resulted in 2.5-fold activation (Fig. 4d). Interestingly, coinducing with both ABA and GA to simultaneously recruit KRAB and VPR also led to 2.5-fold EGFP repression, suggesting that repression from KRAB may be dominant to activation from VPR. We further characterized this system’s ability to switch from an activated to repressed state and vice versa (Fig. 4e). Replacement of either inducer with the other led to an immediate reversal of the transcriptional regulatory effect, and EGFP levels subsequently passed the basal expression level within 48 h. Compared to simple removal of an inducer from individual activator or repressor systems (Fig. 2b and Supplementary Fig. 4b), recruiting an opposing effector accelerated reversal of the initial regulatory effect.

DISCUSSION

Using the ABA and GA chemical-inducible systems, we were able to achieve strong inducible activation of gene expression. Unlike inducible promoter systems and previously generated inducible dCas9s, we detected no leaky activity from our inducible dCas9s.

The inducible dCas9s functioned at levels comparable to direct-fusion dCas9 activators, with added features for reversible and dose-dependent gene regulation. For repression, KRAB–dCas9 acts additively via dCas9 steric hindrance of RNA polymerase and via KRAB-induced repressive chromatin modifications12,13,40. In our dimerization strategy, since dCas9 remains DNA bound in the uninduced state, achieving inducible repression relies on minimizing the steric effect while maximizing the chromatin modification effect. This outcome was achieved by constraining sgRNA binding sites to promoter regions upstream of the transcription start site (TSS). Alternative approaches for inducible repression include using a split dCas9 (refs. 20 and 41) or a caging mechanism42.

Orthogonal dCas9 systems allow simultaneous activation and repression of different genes in one cell. By further pairing each dCas9 with a discrete inducible system, we enable independent temporal control of each gene target. Inducible and orthogonal dCas9-mediated regulation of discrete genes at different timepoints provides new means to dissect the causal factors that govern complex and dynamic processes such as cell differentiation and oncogenesis (Fig. 5). The orthogonal dCas9s also immediately permit functional genomic screens based on combinatorial activation and repression, currently limited to activation or repression only. Furthermore, additional dCas9 orthologs or nuclease-dead mutants of the type V CRISPR protein family Cpf1 (ref. 43) can be integrated into our platform to increase the sets of genes simultaneously manipulated.

Figure 5.

Figure 5

Transcriptome engineering using orthogonal and inducible dCas9 regulators. Inducible dCas9 regulators can be used in combination in cells to achieve orthogonal and multiplexed transcriptome manipulation.

Engineering additional layers of control for individual dCas9 regulators via Boolean logic gates can be useful when manipulating natural gene networks. For example, in the context of gene-based therapeutics, Boolean logic has been used to increase specificity when rewiring natural gene circuits to produce novel functional outputs44,45. Parallel fusion of dimerization domains also enables recruitment of disparate effectors to the same site, as shown by diametric gene regulation. Expanding the method of parallel effector recruitment to other synergistic transcriptional or epigenetic regulators46,47 may allow inducible encoding of combinatorial regulatory functions at a target locus and enable fine-tuned control of both magnitude and permanence of target gene expression.

Several proof-of-principle studies have demonstrated that dCas9-based synthetic gene circuits can operate in mammalian cells34,48,49. In these studies, synthetic circuit regulation was achieved by conditionally expressing sgRNAs from engineered Pol II promoters. A proposed alternative approach is to modulate the activity of dCas9 itself. However, the key challenge in this strategy is developing multiple dCas9 regulators that can act without crosstalk and can be independently regulated21. The ABA- and GA-inducible Sp and Sa dCas9 systems developed in this work satisfy both requirements. The Boolean logic gates generated here also offer a protein-based alternative for processing multiple inputs to produce a functional output.

Synthetic biology aims to build complex biological systems from simple biological parts. In this study, we demonstrate a synthetic biology approach for creating a modular dCas9 system that responds to chemical inputs and produces disparate or orthogonal transcriptional outputs. We envision that our system can be expanded into a ‘plug-and-play’ toolbox to generate a vast array of increasingly complex dCas9 transcriptional regulators, and that it will facilitate engineering the transcriptome to elucidate causal relationships between gene networks and cellular phenotypes.

METHODS

Methods and any associated references are available in the online version of the paper.

ONLINE METHODS

Step-by-step protocol

A step-by-step protocol for dual activation of CD95 and CXCR4 in HEK293T cells using orthogonal inducible dCas9s has been included as a Supplementary Protocol and is available at Protocol Exchange (http://dx.doi.org/10.1038/protex.2016.080).

Plasmid design and construction of inducible dCas9s

Individual constructs for dCas9s, effectors, and sgRNAs used in this study are described in Supplementary Table 2, ordered by figure. Protein domain peptide sequences and sgRNA nucleotide sequences are included in Supplementary Notes 1 and 2 (plasmids no. 84239–84261). New constructs from this study are available on Addgene.

dCas9 fusions

Human codon-optimized Streptococcus pyogenes dCas9 with two C-terminal SV40 nuclear localization signals (NLSs) or Staphylococcus aureus dCas9 with an N-terminal SV40 NLS and C-terminal nucleoplasmin NLS (gift from Feng Zhang, MIT, Addgene plasmid no. 61594)32 was fused at the N terminus to a heterodimerization domain and tagBFP. For the OR gate and diametric dCas9, a second heterodimerization domain (ABI) was fused to the C terminus of dCas9. Expression of dCas9 was driven by a PGK promoter.

Transcriptional effector fusions

The VPR activator22 was assembled by fusing the activation domain of VP64 with the activation domains of p65 (p65AD) and RTA via two GS linkers. An extra SV40 NLS was inserted between VP64 and p65AD. The tripartite sequence was then fused onto the C terminus of a heterodimerization domain. The VP64 activator, KRAB repressor, and GCN4 SunTag scaffold were fused to the C terminus of the heterodimerization domain in an analogous fashion. Expression of the transcriptional effector was driven by a CAG promoter.

Heterodimerization domains.

The following heterodimerization domains were used: ABI–PYL1 (gift from J. Crabtree, Stanford, Addgene plasmid no. 38247)23, GAI–GID1 (gift from T. Inoue, Johns Hopkins, Addgene plasmid nos. 37309, 37305)24, FKBP–FRB (gift from T. Meyer, Stanford, Addgene plasmid nos. 20160, 20148)25, PhyB–PIF and CRY2PHR–CIBN (gift from C. Tucker, U of Colorado, Addgene plasmid nos. 26866, 26889)27, and GI–FKF1 (gift from R. Dolmetsch, Stanford, Addgene plasmid nos. 42499, 42500)28.

Transcriptional effectors and dCas9s were amplified by PCR and cloned onto a PiggyBac vector by Gibson assembly. An IRES-driven puromycin- or zeocin-resistance gene and a WPRE sequence were placed 3′ of the transcriptional effector and 5′ of the dCas9. For the AND gate, the IRES-puro was replaced by a p2A–GID1–ABI fusion. Variants were generated by restriction digest and InFusion (Clontech) cloning.

The sgRNAs were cloned onto a pHR lentiviral U6-based expression vector that coexpressed mCherry, EGFP, or mIFP (infrared fluorescent protein, gift from X. Shu, UCSF) from a CMV promoter. For the OR gate and diametric dCas9, the CMV promoter was used to drive expression of the second heterodimerizer–transcriptional effector cassette followed by an IRES–mCherry. Alternative sgRNA sequences were generated by PCR and introduced by InFusion cloning into a vector digested with BstXI and NotI (New England BioLabs).

Construct optimization

A series of different construct designs using various promoters, heterodimer domain configurations, and plasmid backbones were tested to optimize the activity of the inducible dCas9s (Supplementary Fig. 3 and unpublished data). We noted that the maximal level of activation was determined to occur at high expression levels of the transcriptional effector and low expression levels of the dCas9. To increase expression of the transcriptional effector, a strong constitutive promoter (pCAG) was used to drive transcription, and a WPRE sequence was added to enhance expression. Adding an SV40 polyA transcriptional terminator after the WPRE was found to have disparate effects on different constructs, and the empirically determined best construct was selected in each case. To reduce expression of dCas9, a weak constitutive promoter (pPGK) was selected.

Cell culture and reporter cell line generation

HEK293T cells (Clontech no. 632180) were maintained in Dulbecco’s modified Eagle medium (DMEM) containing 10% FBS, 25 mM D-glucose, and 1.0 mM sodium pyruvate (Life Technologies) in incubators at 37 °C and 5% CO2. Cells were not tested for authenticity and were not routinely tested for mycoplasma contamination. The HEK293T pTRE3G–EGFP and HEK293T pSV40–EGFP reporter lines were generated by transducing cells with lentivirus containing EGFP expressed from a pTRE3G (Clontech) or pSV40 promoter. The HEK293T pSV40–EGFP pTRE3G–mCherry cell line was generated by transducing HEK293T pSV40–EGFP reporter cells with lentivirus expressing mCherry from a pTRE3G promoter. Pure populations of reporter cells were bulk sorted by fluorescence activated cell sorting (FACS) using a BD FACS Aria2 for stable fluorescent marker expression. In the case of HEK293T pTRE3G–EGFP, 1 μg/mL doxycycline was added, and cells were transfected with a transactivator rtTA plasmid (Clontech) for sorting of EGFP+ reporter cells, which were then cultured in the absence of doxycycline after sorting.

Lentivirus production

Lentivirus was generated by transiently transfecting HEK293T cells with the pHR construct of interest, pCMV–dR8.91, and pMD2.G at a ratio of 9:8:1, respectively. Cell media was changed 24 h after transfection. Viral supernatant was collected 48 h post-transfection, passed through a 0.45 μm filter, and concentrated 10× using the Lenti-X Concentrator (Clontech) by centrifugation at 4 °C for 45 min at 1,500× g following incubation overnight at 4 °C.

Stable dCas9 cell line generation

To generate cell lines stably expressing PiggyBac-based dCas9 and sgRNA constructs, reporter cells were seeded at a density of 2 × 105 per well in a 12-well plate and transfected the following day with 0.5 μg PiggyBac plasmid containing dCas9 and 0.2 μg Super PiggyBac Transposase plasmid (Systems Biosciences). Approximately 10 d after transfection, cells were sorted by FACS using a BD FACS Aria2 for stable fluorescent marker expression. Where necessary, cells were transduced with lentivirus containing sgRNA before FACS.

Induction experiments

HEK293T cells were seeded at a density of 3 × 104 per well in 24-well plates (reporter) or 6 × 104 per well in 12-well plates (endogenous gene). For transient transfection experiments, cells were transfected 1 d after seeding with 0.5 μg total of dCas9 and 0.5 μg total of sgRNA per well using TransIT-LT1 transfection reagent (Mirus) at a ratio of 3 μL transfection reagent per 1 μg plasmid. Transfected cells were induced 1 d after transfection by treatment with 100 μM abscisic acid (ABA, Sigma), 10 μM gibberellic acid acetoxymethyl ester (GA, Santa Cruz), 1 μM rapamycin analog (A/C Heterodimerizer, Clontech), or they were placed in light. For red light induction, 15 μM phycocyanobilin (Santa Cruz) was added to cells 3 h before the start of induction, and 10 μW 650 nm light was pulsed in cycles of 20 s ON, 60 s OFF. For blue light, 50 μW 450 nm light was pulsed in cycles of 500 ms ON, 5 s OFF. Stable cell lines were instead induced 1 day after seeding.

Cells were collected after 48 h induction for activation experiments, after 5 d induction for repression experiments, or after 7 d induction for timecourse experiments. For repression experiments, cells were passaged after 48 h induction and treated with fresh inducer. For timecourse experiments, cells were passaged after 48 h and 5 d induction and treated with fresh inducer. To remove inducer during timecourses, inducer-treated media was aspirated and replaced with fresh media. No additional washing was performed when removing inducer to minimize detachment and loss of low-confluence cells.

Fluorescence and immunostaining assays using flow cytometry analysis

To analyze fluorescent protein expression, cells were dissociated using 0.05% Trypsin EDTA (Life Technologies) and analyzed by flow cytometry on a BD LSRII. To analyze surface marker expression, cells were instead dissociated using the nonenzymatic reagent Versene (Life Technologies), then stained in 10% FBS in PBS for 1 h at room temperature. Antibodies were purchased from BioLegend (nos. 306510, 400322, 305607, 400111). CXCR4 antibody and isotype were used at 0.4 μg/mL, and CD95 antibody and isotype were used at 0.5 μg/mL.

Flow cytometry data was analyzed using FlowJo. At least 10,000 cells were analyzed for each sample. For stably integrated cell lines, cells were gated for positive fluorescent marker expression corresponding to construct expression. For 48 h transfection experiments, cells were gated for positive dCas9 (tagBFP) expression, then further gated for the highest 50% of sgRNA (mCherry, EGFP, or mIFP) expressing cells. For 5 d transfection experiments, cells were gated for the highest 20% of sgRNA expression on account of dilution of transfected plasmids. The mean intensity of the reporter fluorescent protein (EGFP or mCherry) or immunofluorescent antibody (APC-CXCR4 or PE-CD95) was measured. When calculating the fold change of endogenous proteins, we subtracted the fluorescence level of isotype-stained wild-type HEK293T cells from the measured fluorescence of samples.

Experimental design and statistics

Data are displayed as individual points or as mean ± s.d., with sample size indicated in figure legends. No sample size estimates were performed, and the sample sizes used in this study are consistent with those used by similar genome editing and gene regulation studies. No samples were excluded from analysis. No randomization or blinding was performed.

Statistical analysis was performed using SPSS Statistics 21 (IBM). Equal variance between populations was not assumed. To account for unequal variance among conditions, Welch’s two-sided t-test was performed when comparing two conditions, and Welch’s ANOVA was performed followed by Games–Howell post hoc tests when comparing more than two conditions with each other.

Supplementary Material

Supplemenal Figures

Acknowledgments

The authors thank the members of the Qi and Lim labs for advice and helpful discussions and the Stanford Shared FACS Facility for technical support. The authors also thank X. Shu (UCSF), F. Zhang (MIT), G. Crabtree (Stanford), T. Inoue (Johns Hopkins), T. Meyer (Stanford), C. Tucker (U of Colorado), and R. Dolmetsch (Stanford) for constructs used in this study. L.S.Q. acknowledges support from the NIH Office of the Director (OD) and the National Institute of Dental & Craniofacial Research (NIDCR). Y.G. acknowledges support from the Stanford Cancer Biology Graduate Program and the NSF GRFP fellowship. X.X. acknowledges the support from the Helen Hay Whitney Foundation postdoctoral fellowship. This work was supported by DP5 OD017887 (L.S.Q.), NIH R01 DA036858 (L.S.Q. and W.A.L.), NIH P50 GM081879 (W.A.L.), and the Howard Hughes Medical Institute (W.A.L.).

Footnotes

Note: Any Supplementary Information and Source Data files are available in the online version of the paper.

AUTHOR CONTRIBUTIONS

L.S.Q. and W.A.L. conceived of the research; Y.G. and X.X. designed the study; Y.G., X.X., S.W., and E.J.C. performed the experiments; Y.G., X.X., S.W., and L.S.Q. analyzed the data; Y.G., X.X., and L.S.Q. wrote the manuscript with input from all authors.

COMPETING FINANCIAL INTERESTS

The authors declare no competing financial interests.

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