Local complement activation in aqueous humor in patients with age-related macular degeneration

Abstract

Purpose

To investigate complement activation in aqueous humor and in plasma of patients with neovascular age-related macular degeneration (nAMD).

Patients and methods

Aqueous humor and EDTA-plasma of 31 nAMD patients and 30 age-matched controls was collected. The levels of the complement factor 3 (C3), the regulators factor H (FH), and factor I (FI), and of the complement activation products Ba, C3a, and the terminal complement complex (sC5b-9) were measured. Associations between complement levels and phenotype were determined using Mann–Whitney U-test.

Results

In plasma, no significant differences were found between the nAMD group and the control group. In aqueous humor, significantly increased levels of Ba (P=0.002), and C3a (P=0.002) indicate local complement activation in nAMD patients and a trend for a concomitant upregulation of the complement regulators FH (P=0.02) and FI (P=0.04).

Conclusions

Our findings provide strong evidence for a local complement dysregulation in nAMD patients.

Introduction

Age-related macular degeneration (AMD) is a major cause of blindness in the elderly population. In the early stages, deposits of cellular debris and inflammatory material accumulate between retinal pigment epithelium and Bruch's membrane. Late AMD is characterized by the development of either geographic atrophy or neovascular AMD (nAMD). A dysregulation of the alternative pathway of the complement cascade has been postulated to be the key to the pathogenesis of AMD.^{1, 2, 3, 4} Various complement proteins, their activation products, and regulators have been identified in retinal deposits of AMD patients. Several genes that are associated with AMD encode for proteins of the complement system, including the components component 3 (C3), component 2 (C2), and factor B (FB), as well as the regulators factor H (FH) and factor I (FI).^{1, 5, 6, 7, 8, 9, 10, 11, 12}

Although AMD disease manifestation is localized in the eye, a slight systemic elevation of complement components and activation products in AMD patients were found for FB and factor D as well as for 3a, C5a, C3d, and Ba, respectively.^{13, 14} However, little is known about the extent and relationship between systemic and local complement dysregulation in AMD. In this study, we analyzed various complement proteins, their activation products and regulators in aqueous humor and in EDTA-plasma of patients with and without nAMD.

Methods

Study population

In this prospective study, 61 participants (31 nAMD patients, 30 age-matched controls) from the Department of Ophthalmology, University Hospital of Cologne were included. The study was performed in accordance with the tenets of the Declaration of Helsinki and the Medical Research Involving Human Subjects Act (WMO) and was approved by the local ethics committee of the University Hospital in Cologne. Written informed consent was obtained from all participants.

Patient staging was performed by grading of retinal images including stereo fundus photographs in controls and fundus photographs, fluorescein angiograms, and spectral domain optical coherence tomograms in nAMD patients. Controls had no drusen in both eyes, nAMD was classified by active choroidal neovascularisation secondary to AMD in the study eye at the time point of collection of samples.

Inclusion and exclusion criteria

Included were patients with nAMD undergoing IVI with anti-VEGF agents (bevazizumab, ranibizumab, or aflibercept) as well as controls undergoing cataract surgery. Exclusion criteria were any previous ophthalmic surgery during the last 6 months. In the nAMD group, IVI with anti-VEGF agents (bevazizumab, ranibizumab, or aflibercept) were allowed to be injected more than 4 weeks ago and therefore were not an exclusion criterion. Additional exclusion criteria were ocular malignoma, diseases resulting in inflammation or hemorrhage in the study eye and any systemic diseases potentially inducing the complement system such as diabetes, autoimmune diseases, cancer, or infectious diseases.

Collection of samples

Undiluted samples of aqueous humor were acquired before any other surgical manipulation of the cataract operation and before IVI. An aliquot of 0.1 ml of aqueous humor was collected via limbal paracentesis using a 30-gauge needle connected to an insulin syringe. Care was taken not to contaminate the sample with blood. Samples were transferred to sterile polypropylene tubes immediately after collection and stored at −80 °C until analysis was performed.

Measurements of complement levels

C3, FH,¹⁵ FI,¹⁶ and sC5b-9¹⁷ were measured by ELISA as previously described. C3a (Progen, Heidelberg, Germany) and Ba (Quidel, San Diego, CA, USA) were measured by ELISA following the manufacturer's protocols. All assays were adapted for analysis in aqueous humor.

Statistical analysis

All calculations were performed using SPSS software version 22.0 (IBM Software and Systems, Armonk, NY, USA). Mann–Whitney U-test (two-sided) was used to compare complement levels in the nAMD group and the control group. Correlations in nAMD cases between number of IVIs and complement levels and between local and systemic complement levels were performed using Spearman correlation (two-sided). Bonferroni correction was performed due to multiple testing and P-values <0.0045 were considered statistically significant. As this is a pilot study and no boundary values for local complement values exist, power calculations could not be performed previously.

Results

In the study, 30 controls and 31 patients with nAMD were included. Age did not differ significantly between both groups (P=0.09; controls: mean±SD 73.3±5.9 years vs AMD patients 75.5±4.8 years). In the control group, 21 were male, the nAMD group comprised 11 male participants. Mean number of previous IVIs in the nAMD group was 5.4 (range 0–14 IVIs). Complement protein concentrations did not correlate with the number of previous IVIs (Table 1). Concentrations for all parameters for nAMD patients and controls are summarized in Table 2.

Table 1. Influence of number of IVIs in the group of patients with neovascular age-related macular degeneration on complement levels.

Parameter	Number of IVIs (Spearman correlation)
Plasma
SC5b-9 (ng/ml)	R=0.05, P=0.83
Ba (n/ml)	R=0.09, P=0.69
FH (μg/ml)	R=−0.24, P=0.27
FI (μg/ml)	R=−0.38, P=0.08
C3 (μg/ml)	R=−0.26, P=0.22
C3a (ng/ml)	R=0.22, P=0.33
Aqueous humor
Ba (ng/ml)	R=−0.08, P=0.72
FH (μg/ml)	R=−0.09, P=0.71
FI (μg/ml)	R=−0.32, P=0.17
C3 (μg/ml)	R=−0.37, P=0.11
C3a (ng/ml)	R=−0.09, P=0.68

Table 2. Median complement factor levels in plasma and aqueous humor in nAMD patients and controls.

Parameter	Median (1st–3rd quartile)		P-value
	Control	nAMD
Plasma
SC5b-9 (ng/ml)	151 (113–199)	175 (121–224)	0.17
Ba (n/ml)	710 (535–851)	738 (645–906)	0.20
FH (μg/ml)	289 (252–368)	298 (270–367)	0.58
FI (μg/ml)	27.5 (23–35)	26.5 (23–31)	0.42
C3 (μg/ml)	985 (720–1170)	895 (655–1027)	0.11
C3a (ng/ml)	292 (239–410)	298 (218–422)	0.89
Aqueous humor
Ba (ng/ml)	11.5 (7.0–21.5)	22.8 (16.2–28.5)	0.002*
FH (μg/ml)	0.13 (0.09–0.21)	0.16 (0.13–0.37)	0.02
FI (μg/ml)	0.11 (0.08–0.20)	0.17 (0.11–0.28)	0.04
C3 (μg/ml)	0.72 (0.29–1.33)	0.78 (0.47–2.22)	0.25
C3a (ng/ml)	21.0 (13.8–26.0)	33.0 (24.3–37.5)	0.002*

Abbreviation: nAMD, neovascular AMD. Mann–Whitney U-test, *statistically significant (after Bonferroni correction, if P<0.0045).

In plasma, none of the tested complement parameters showed a significant difference between nAMD patients and controls (Table 2). In aqueous humor, nAMD patients had significantly higher levels of complement activation markers Ba and C3a, but also a trend for an upregulation of the regulatory proteins FH and FI (Table 2). Local and systemic complement levels showed slight positive correlation for Ba and C3 (Table 3).

Table 3. Spearman correlation of plasma and aqueous humor levels per parameter.

Parameter	R	P-value
Ba	0.53	0.00003
FH	0.34	0.03
FI	0.43	0.006
C3	0.44	0.001
C3a	0.25	0.10

Discussion

Increased complement activation has been suggested to be one of the main pathomechanisms in AMD. This hypothesis is supported by large genetic association studies.⁷ All relevant complement genes associated with activation and regulation of the complement activation pathways are expressed locally in the human RPE-choroid complex.¹⁸

In this study, we analyzed complement C3, activation markers and regulatory proteins in aqueous humor and in plasma of patients with nAMD and healthy controls. Although plasma concentrations of complement proteins showed no significant difference between both groups, activation products C3a and Ba were significantly elevated in aqueous humor of nAMD patients. In addition, there was a trend for elevated regulators FH and FI in aqueous humor in nAMD.

Thus, complement activation was significantly increased despite a trend for an upregulation of complement inhibitors. Therefore, inhibitors could not compensate for an increased yet unidentified stimulus for complement activation.

Although systemic complement activation in plasma was slightly increased in AMD patients compared with controls, the difference was statistically not significant. This was in clear contrast to the very pronounced increase in complement activation in aqueous humor.

In plasma, other studies also found increased plasma complement levels in patients with AMD.^{13, 14} The differences were statistically significant due to slightly higher median values in a large number of samples with a big overlap in the range of values between both groups. This is not relevant on the individual level because there is a large variation in systemic complement levels. In addition, various factors and common diseases such as diabetes have influence on systemic complement activation and impede the interpretation.

Between local and systemic complement levels, we could find slight correlations for Ba and C3. Therefore, for some components there might be a low influence on systemic complement levels due to local complement activation in AMD patients.

Overall, this data supports the notion that there is a significant dysregulation of the complement cascade in AMD. We could demonstrate that this is essentially a local event in the eye more than in the systemic circulation.

Our study comprised a relatively small number of samples and reference values of the measured intraocular complement levels do not exist yet which is mainly due to the limited access to aqueous humor samples. Number of injections of anti-VEGF agents >4 weeks before collection of samples did not correlate with complement levels but an influence of IVIs cannot be ruled out. Hence, larger cohorts of therapy-naïve patients and controls should be analyzed in further studies. Only data on patients with nAMD but not on dry AMD were available for this study. Based on measurements of plasma samples with small differences in complement activation between both wet and dry AMD, and controls it is likely that ocular complement activation is also increased in dry AMD.¹⁹

In conclusion, our study clearly demonstrates increased complement activation in aqueous humor of nAMD patients supporting the hypothesis of a local dysregulation of the complement system in AMD.