Figure 6. leon mutant phenotypes suppressed by reductions of gene dosages.

(A) Immunostaining images show suppression of Cact expansion in leon¹/19-2 by dlg^X1-2, and suppression of GluRIIA enlargement by dpak⁶, pll²⁵ and dl^H. (B) Bar graphs show means ± SEM of Cact-positive zone thickness and GluRIIA intensity and diameter. (C) Immunostaining images of NMJs for Dlg and GluRIIA show suppression of GluRIIA enlargement in cact¹³/+; leon¹/19-2 as compared to leon¹/19-2. (D) Bar graphs show means ± SEM of Dlg-positive zone thickness and GluRIIA intensity and diameter. Comparisons to leon¹/19-2 were assessed by Student’s t tests with n.s. indicating no significance, * for p<0.05, ** for p<0.01 and *** for p<0.001. Scale bar, 5 μm. The detail statistic numbers are in Supplementary file 1. (E) Western blots show enhanced signals of Dlg, dPak and Cact in leon¹/19-2 in comparison to w¹¹¹⁸. α-Tub as control.

DOI: http://dx.doi.org/10.7554/eLife.26886.013

Figure 6—figure supplement 1. leon mutant phenotypes suppressed by knockdown of postsynaptic proteins.

(A) Examining expressions of pll in da-GAL4-driven pllRNAi by RT-PCR. The mRNA levels of pll decreased in 24B>pllRNAi as compared to GAL4 control. The mRNA expression of Rpl19 serves as control. (B) Postsynaptic expression of mCD8GFP by 24B-GAL4 shows SSR enriched GFP signals with magnification on the right. Scale bars: left, 10 μm and right, 5 μm. (C) Immunostaining images of NMJs for GFP and GluRIIA show suppression of mCD8GFP-labeled SSR in leon¹/19-2 by RNAi transgenes for dlg and dpak, and suppression of GluRIIA enlargement by RNAi transgenes for dpak, pll, dl-B and cact. Scale bar, 5 μm. (D) Bar graphs show means ± SEM of GFP-positive zone thickness and GluRIIA intensity and diameter. Comparisons to leon¹/19-2 were assessed by Student’s t tests with n.s. indicating no significance and *** for p<0.001. The detail statistic numbers are in Supplementary file 1.

DOI: http://dx.doi.org/10.7554/eLife.26886.014