Table 2. Primers designed for miR-21, Bcl-2, Bax, VEGF and GAPDH.
PCR primerssequence | Forward (5′-3′) | Reverse (5′-3′) |
---|---|---|
miR-21 | CGGGATCCAGCCACTACCAAGGCATGTT | CGGAATTCAACCACGACTAGAGGCTGAC |
Bcl-2 | GAAACAGATGTCCCTACCAACCAGA | TCAGCATGGCTCAAAGTGCAG |
Bax | CCAGTTGAAGTTGCCGTCAGAA | GCGAGTGTCTCAAGCGCATC |
VEGF | TGTGCGGGCTGCTGCAATGAT | TGTGCTGGCTTTGGTGAGGTTTGA |
GAPDH | AGGGGCCATCCACAGTCTTC | AGAAGGCTGGGGCTCATTTG |
Note: Bcl-2, B-cell lymphoma-2 B; Bax, Bcl-2 Associated X Protein B; VEGF, vascular endothelial growth factor; GAPDH, glyceraldehyde-phosphate dehydrogenase; miR-21, microRNA-21.