Skip to main content
. 2017 Mar 22;8(17):29328–29345. doi: 10.18632/oncotarget.16440

Figure 7. Z-LIG decreased MTA1 expression and recruitment onto the ERα promoter in ERα− breast cancer cells.

Figure 7

(A) Expression of MTA1 was detected by Western blotting in MDA-MB-231, Hs578t and MDA-MB-453 cells after treated with Z-LIG for 72 h. (B) ChIP analysis showing recruitment of MTA1 onto ERpro315 of the ERα promoter. MDA-MB-231 cells were treated with Z-LIG (50 μM) for 72 h. DNA fragments that immunoprecipitated by normal IgG or anti-MTA1 antibodies were amplified by PCR using primers for ERpro315. (C) Expression of MTA1 was detected by Western blotting after MDA-MB-231 cells were transfected with pcMV-vector or pcMV-MTA1 for 24 h and then treated with Z-LIG (50 μM) for 72 h. (D) The cell viability was detected by SRB assay after MDA-MB-231 cells were transfected with pcMV-vector or pcMV-MTA1, and then treated with or without Z-LIG (50 μM) combined with TAM (5 μM) for 72 h. (E) Colony formation assay was performed in MDA-MB-231 cells that first transfected with pcMV-vector or pcMV-MTA1, and then treated with combinatorial Z-LIG (25 μM) and TAM (2.5 μM) and allowed to grow for two weeks before stained with 0.005% crystal violet. Values represent mean ± SD. The blots or images were a representative of three independent experiments. *p< 0.05, **p< 0.01 compared with control.