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. 1990 Aug;87(15):5667–5671. doi: 10.1073/pnas.87.15.5667

Complete sequence of human vinculin and assignment of the gene to chromosome 10.

P A Weller 1, E P Ogryzko 1, E B Corben 1, N I Zhidkova 1, B Patel 1, G J Price 1, N K Spurr 1, V E Koteliansky 1, D R Critchley 1
PMCID: PMC54388  PMID: 2116004

Abstract

We have determined the complete sequence of human vinculin, a cytoskeletal protein associated with cell-cell and cell-matrix junctions. Comparison of human and chicken embryo vinculin sequences shows that both proteins contain 1066 amino acids and exhibit a high level of sequence identity (greater than 95%). The region of greatest divergence falls within three 112-amino acid repeats spanning residues 259-589. Interestingly, nematode vinculin lacks one of these central repeats. The regions of human vinculin that are N- and C-terminal to the repeats show 54% and 61% sequence identity, respectively, to nematode vinculin. Southern blots of human genomic DNA hybridized with short vinculin cDNA fragments indicate that there is a single vinculin gene. By using a panel of human-rodent somatic cell hybrids, the human vinculin gene was mapped to chromosome 10q11.2-qter.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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