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. 1990 Aug;87(15):5710–5714. doi: 10.1073/pnas.87.15.5710

Gene expression of fibroblast growth factors in human gliomas and meningiomas: demonstration of cellular source of basic fibroblast growth factor mRNA and peptide in tumor tissues.

J A Takahashi 1, H Mori 1, M Fukumoto 1, K Igarashi 1, M Jaye 1, Y Oda 1, H Kikuchi 1, M Hatanaka 1
PMCID: PMC54397  PMID: 2377607

Abstract

The growth autonomy of human tumor cells is considered due to the endogenous production of growth factors. Transcriptional expression of candidates for autocrine stimulatory factors such as basic fibroblast growth factor (FGF), acidic FGF, and transforming growth factor type beta were determined in human brain tumors. Basic FGF was expressed abundantly in 17 of 18 gliomas, 20 of 22 meninglomas, and 0 of 5 metastatic brain tumors. The level of mRNA expression of acidic FGF in gliomas was significant. In contrast, transforming growth factor type beta 1 was expressed in all the samples investigated. The mRNA for basic FGF and its peptide were localized in tumor cells in vivo by in situ hybridization and immunohistochemistry, showing that basic FGF is actually produced in tumor cells. Our results suggest that tumor-derived basic FGF is involved in the progression of gliomas and meningiomas in vivo, whereas acidic FGF is expressed in a tumor origin-specific manner, suggesting that acidic FGF works in tandem with basic FGF in glioma tumorigenesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abraham J. A., Mergia A., Whang J. L., Tumolo A., Friedman J., Hjerrild K. A., Gospodarowicz D., Fiddes J. C. Nucleotide sequence of a bovine clone encoding the angiogenic protein, basic fibroblast growth factor. Science. 1986 Aug 1;233(4763):545–548. doi: 10.1126/science.2425435. [DOI] [PubMed] [Google Scholar]
  2. Abraham J. A., Whang J. L., Tumolo A., Mergia A., Friedman J., Gospodarowicz D., Fiddes J. C. Human basic fibroblast growth factor: nucleotide sequence and genomic organization. EMBO J. 1986 Oct;5(10):2523–2528. doi: 10.1002/j.1460-2075.1986.tb04530.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Crumley G. R., Howk R., Ravera M. W., Jaye M. Multiple polyadenylation sites downstream from the human aFGF gene encoding acidic fibroblast growth factor. Gene. 1989 Dec 28;85(2):489–497. doi: 10.1016/0378-1119(89)90443-5. [DOI] [PubMed] [Google Scholar]
  4. Derynck R., Jarrett J. A., Chen E. Y., Eaton D. H., Bell J. R., Assoian R. K., Roberts A. B., Sporn M. B., Goeddel D. V. Human transforming growth factor-beta complementary DNA sequence and expression in normal and transformed cells. Nature. 1985 Aug 22;316(6030):701–705. doi: 10.1038/316701a0. [DOI] [PubMed] [Google Scholar]
  5. Ferrara N., Ousley F., Gospodarowicz D. Bovine brain astrocytes express basic fibroblast growth factor, a neurotropic and angiogenic mitogen. Brain Res. 1988 Oct 18;462(2):223–232. doi: 10.1016/0006-8993(88)90550-1. [DOI] [PubMed] [Google Scholar]
  6. Folkman J., Klagsbrun M. Angiogenic factors. Science. 1987 Jan 23;235(4787):442–447. doi: 10.1126/science.2432664. [DOI] [PubMed] [Google Scholar]
  7. Gospodarowicz D., Ferrara N., Schweigerer L., Neufeld G. Structural characterization and biological functions of fibroblast growth factor. Endocr Rev. 1987 May;8(2):95–114. doi: 10.1210/edrv-8-2-95. [DOI] [PubMed] [Google Scholar]
  8. Gospodarowicz D., Handley H. H. Stimulation of division of Y1 adrenal cells by a growth factor isolated from bovine pituitary glands. Endocrinology. 1975 Jul;97(1):102–107. doi: 10.1210/endo-97-1-102. [DOI] [PubMed] [Google Scholar]
  9. Gospodarowicz D., Neufeld G., Schweigerer L. Fibroblast growth factor: structural and biological properties. J Cell Physiol Suppl. 1987;Suppl 5:15–26. doi: 10.1002/jcp.1041330405. [DOI] [PubMed] [Google Scholar]
  10. Hsu S. M., Soban E. Color modification of diaminobenzidine (DAB) precipitation by metallic ions and its application for double immunohistochemistry. J Histochem Cytochem. 1982 Oct;30(10):1079–1082. doi: 10.1177/30.10.6182185. [DOI] [PubMed] [Google Scholar]
  11. Jaye M., Lyall R. M., Mudd R., Schlessinger J., Sarver N. Expression of acidic fibroblast growth factor cDNA confers growth advantage and tumorigenesis to Swiss 3T3 cells. EMBO J. 1988 Apr;7(4):963–969. doi: 10.1002/j.1460-2075.1988.tb02902.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kurokawa T., Sasada R., Iwane M., Igarashi K. Cloning and expression of cDNA encoding human basic fibroblast growth factor. FEBS Lett. 1987 Mar 9;213(1):189–194. doi: 10.1016/0014-5793(87)81489-8. [DOI] [PubMed] [Google Scholar]
  13. Libermann T. A., Friesel R., Jaye M., Lyall R. M., Westermark B., Drohan W., Schmidt A., Maciag T., Schlessinger J. An angiogenic growth factor is expressed in human glioma cells. EMBO J. 1987 Jun;6(6):1627–1632. doi: 10.1002/j.1460-2075.1987.tb02410.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mori H., Maki M., Oishi K., Jaye M., Igarashi K., Yoshida O., Hatanaka M. Increased expression of genes for basic fibroblast growth factor and transforming growth factor type beta 2 in human benign prostatic hyperplasia. Prostate. 1990;16(1):71–80. doi: 10.1002/pros.2990160108. [DOI] [PubMed] [Google Scholar]
  15. Murphy P. R., Myal Y., Sato Y., Sato R., West M., Friesen H. G. Elevated expression of basic fibroblast growth factor messenger ribonucleic acid in acoustic neuromas. Mol Endocrinol. 1989 Feb;3(2):225–231. doi: 10.1210/mend-3-2-225. [DOI] [PubMed] [Google Scholar]
  16. Neufeld G., Mitchell R., Ponte P., Gospodarowicz D. Expression of human basic fibroblast growth factor cDNA in baby hamster kidney-derived cells results in autonomous cell growth. J Cell Biol. 1988 Apr;106(4):1385–1394. doi: 10.1083/jcb.106.4.1385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sato Y., Murphy P. R., Sato R., Friesen H. G. Fibroblast growth factor release by bovine endothelial cells and human astrocytoma cells in culture is density dependent. Mol Endocrinol. 1989 Apr;3(4):744–748. doi: 10.1210/mend-3-4-744. [DOI] [PubMed] [Google Scholar]
  18. Schweigerer L., Neufeld G., Mergia A., Abraham J. A., Fiddes J. C., Gospodarowicz D. Basic fibroblast growth factor in human rhabdomyosarcoma cells: implications for the proliferation and neovascularization of myoblast-derived tumors. Proc Natl Acad Sci U S A. 1987 Feb;84(3):842–846. doi: 10.1073/pnas.84.3.842. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Seno M., Iwane M., Sasada R., Moriya N., Kurokawa T., Igarashi K. Monoclonal antibodies against human basic fibroblast growth factor. Hybridoma. 1989 Apr;8(2):209–221. doi: 10.1089/hyb.1989.8.209. [DOI] [PubMed] [Google Scholar]
  20. Shing Y., Folkman J., Haudenschild C., Lund D., Crum R., Klagsbrun M. Angiogenesis is stimulated by a tumor-derived endothelial cell growth factor. J Cell Biochem. 1985;29(4):275–287. doi: 10.1002/jcb.240290402. [DOI] [PubMed] [Google Scholar]
  21. Sternfeld M. D., Hendrickson J. E., Keeble W. W., Rosenbaum J. T., Robertson J. E., Pittelkow M. R., Shipley G. D. Differential expression of mRNA coding for heparin-binding growth factor type 2 in human cells. J Cell Physiol. 1988 Aug;136(2):297–304. doi: 10.1002/jcp.1041360212. [DOI] [PubMed] [Google Scholar]
  22. Tsuboi K., Hirayoshi K., Takeuchi K., Sabe H., Shimada Y., Ohshio G., Tobe T., Hatanaka M. Expression of the c-myc gene in human gastrointestinal malignancies. Biochem Biophys Res Commun. 1987 Jul 31;146(2):699–704. doi: 10.1016/0006-291x(87)90585-7. [DOI] [PubMed] [Google Scholar]

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