FIG 5 .

Functional bias among mutations observed in prolonged stationary phase. (A) The top plot shows deviation (log₂ fold change) from the number of mutations expected in each functional category based on its relative size (total nucleotides) in the genome. The bottom plot shows corresponding −log₁₀ P values (adjusted for multiple testing with Holm-Bonferroni method) for over- and underrepresentation. The “Regulation” category appears to be overrepresented in prolonged stationary phase; no such significant bias was observed for spontaneous mutations under the neutral mutation accumulation experiment of Lee et al. (21). (B) Binary matrix for genes mutated in four replicates; gray marks their presence. The genes highlighted in blue type have regulatory functions.