After publication of this article (Górska et al. 2016), we noticed that the original version, unfortunately, contained mistakes. We inadvertently inserted the wrong spectra for Figures 1, 3 and 4 and an incorrect version of Table II. Correct versions are provided below. In addition, in the Results (NMR analysis of L919/B) section of the above-mentioned article, after the statement “The structure of the pentasaccharide repeating unit of L919/B, as determined herein, is shown in Figure 4.” we erroneously omitted the following statement: “This structure is virtually identical to that of the polysaccharide L900/3 isolated from L. rhamnosus LOCK 0900 (Górska et al. 2014).”.
Fig. 1.
Selected parts of the 1H-13C HSQC nuclear magnetic resonance (NMR) spectrum of L919/A. This figure is available in black and white in print and in color at Glycobiology online.
Fig. 3.
Selected parts of the 1H-13C HSQC nuclear magnetic resonance (NMR) spectrum of L919/B.
Fig 4.
The structure of the pentasaccharide repeating unit of L919/B.
Table II.
1H and 13C NMR chemical shifts and selected inter-residue connectivities obtained for the anomeric protons of L919/B from Lactobacillus casei LOCK 0919
| Sugar residue | Chemical shifts 1H, 13C (ppm) | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| H1 | H2 | H3 | H4 | H5 | H6 | H6′ | CH3CO | ||
| C1 | C2 | C3 | C4 | C5 | C6 | ||||
| A | →6)-β-d-ManpNAc-(1→ | 5.019 | 4.432 | 3.74 4 | 3.458 | 3.261 | 4.010 | 3.725 | 1.932 |
| 98.61 | 53.02 | 71.76 | 66.83 | 75.62 | 69.41 | 22.10 | |||
| B | →3,4)-α-d-Galp-(1→ | 4.809 | 3.802 | 3.954 | 4.257 | 4.063 | 3.682 | ||
| 98.22 | 67.86 | 77.39 | 75.55 | 69.91 | 61.03 | ||||
| C | →3)-β-d-GalpNAc-(1→ | 4.651 | 3.974 | 3.768 | 4.021 | 3.561 | 3.704 | 1.947 | |
| 103.34 | 51.51 | 80.34 | 67.88 | 74.68 | 60.31 | 21.91 | |||
| D | →4,6)-β-d-Glcp-(1→ | 4.456 | 3.285 | 3.362 | 3.519 | 3.388 | 3.952 | 3.651 | |
| 104.61 | 72.78 | 75.68 | 73.80 | 75.31 | 64.80 | ||||
| E | β-d-Glcp-(1→ | 4.372 | 3.261 | 3.374 | 3.270 | 3.377 | 3.628 | 3.801 | |
| 102.51 | 72.90 | 75.52 | 69.24 | 75.88 | 60.61 | ||||
| P 2.6 ppm | |||||||||
| Selected inter-residue NOESY and 3JH,C (HMBC) connectivities from the anomeric protons of the isolated | |||||||||
| Sugar residue | H-1 δH (ppm) | Connectivity δH (ppm) | Inter-residue atom/residue | Connectivity δC (ppm) | Inter-residue atom/residue | ||||
| A | →6)-β-d-ManpNAc-(1→ | 5.019 | 4.257 | H-4 of B | 75.55 | C-4 of B | |||
| 53.02 | C-2 of A | ||||||||
| B | →3,4)-α-d-Galp-(1→ | 4.809 | 3.519 | H-4 of D | 69.91 | C-5 of B | |||
| 4.063 | H-5 of B | ||||||||
| 3.682 | H-6 of B | ||||||||
| C | →3)-β-d-GalpNAc-(1→ | 4.651 | 3.954 | H-3 of B | 77.39 | C-3 of B | |||
| D | →4,6)-β-d-Glcp-(1→ | 4.456 | 3.768 | H-3 of C | 80.34 | C-3 of C | |||
| E | β-d-Glcp-(1→ | 4.372 | 4.010, 3.722 | H-6 of A | 69.41 | C-6 of A | |||
| P | 64.80 | C-6 of D | |||||||
Spectra were obtained for 2H2O solutions at 25°C, and acetone (δH 2.225, δC 31.05 ppm) was used as an internal reference.
We apologize for the oversight and possible misunderstanding.
References
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