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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Genesis. 2017 Apr 22;55(5):10.1002/dvg.23029. doi: 10.1002/dvg.23029

Fig. 4. Expression of Nv-trbl.

Fig. 4

a-d White, DAPI (DNA). Fig a′-d′ green, Nv-trbl mRNA. (a-a′, b-b′) Male and female embryos at division cycle 12. Zygotic Nv-trbl mRNA is expressed at the anterior presumptive head and along broad bands across the anterior-posterior axis. (c-c′) Embryo at the end of division cycle 12, just prior to gastrulation. Zygotic Nv-trbl mRNA is upregulated in the ventral ectoderm. (d-d′) Embryo at the onset of gastrulation. Zygotic Nv-trbl flanks the mesoderm. (e) qRT-PCR of Nv-trbl normalized to housekeeping gene, Rpo49. Maternal Nv-trbl levels decrease throughout the early syncytial stages and zygotic Nv-trbl expression is upregulated in 4-6 hour embryos. 0-2 hours is representative of pre-blastoderm embryos; 2-4 hours is representative of middle blastoderm embryos; 4-6 hours is representative of late blastoderm and cellularized embryos.