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. 1990 Aug;87(16):6044–6048. doi: 10.1073/pnas.87.16.6044

Mutational analysis of the N terminus of the protein of maize transposable element Ac.

M G Li 1, P Starlinger 1
PMCID: PMC54468  PMID: 2166942

Abstract

Mutations of transposable element Ac were tested for their capability to excise themselves from their location autonomously, to be excised by an active Ac, or to act in trans in the excision of an Ac delta element. Removal of 101 amino acids from the N terminus of the Ac protein does not decrease excision. A cis-acting site between base pairs 186 and 207 is important for excision by the wild-type protein but is not necessary for excision by the truncated protein. Improvement of the sequence context of the first AUG does not have a significant effect. Mutations in a small open reading frame of Ac encoding a 102-amino acid protein do not visibly alter excision frequency.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker B., Coupland G., Fedoroff N., Starlinger P., Schell J. Phenotypic assay for excision of the maize controlling element Ac in tobacco. EMBO J. 1987 Jun;6(6):1547–1554. doi: 10.1002/j.1460-2075.1987.tb02399.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baker B., Schell J., Lörz H., Fedoroff N. Transposition of the maize controlling element "Activator" in tobacco. Proc Natl Acad Sci U S A. 1986 Jul;83(13):4844–4848. doi: 10.1073/pnas.83.13.4844. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baumann G., Raschke E., Bevan M., Schöffl F. Functional analysis of sequences required for transcriptional activation of a soybean heat shock gene in transgenic tobacco plants. EMBO J. 1987 May;6(5):1161–1166. doi: 10.1002/j.1460-2075.1987.tb02349.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coupland G., Baker B., Schell J., Starlinger P. Characterization of the maize transposable element Ac by internal deletions. EMBO J. 1988 Dec 1;7(12):3653–3659. doi: 10.1002/j.1460-2075.1988.tb03246.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Coupland G., Plum C., Chatterjee S., Post A., Starlinger P. Sequences near the termini are required for transposition of the maize transposon Ac in transgenic tobacco plants. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9385–9388. doi: 10.1073/pnas.86.23.9385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jones J. D., Carland F. M., Maliga P., Dooner H. K. Visual detection of transposition of the maize element activator (ac) in tobacco seedlings. Science. 1989 Apr 14;244(4901):204–207. doi: 10.1126/science.244.4901.204. [DOI] [PubMed] [Google Scholar]
  7. Kozak M. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 1987 Oct 26;15(20):8125–8148. doi: 10.1093/nar/15.20.8125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kozak M. Effects of intercistronic length on the efficiency of reinitiation by eucaryotic ribosomes. Mol Cell Biol. 1987 Oct;7(10):3438–3445. doi: 10.1128/mcb.7.10.3438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kozak M. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell. 1986 Jan 31;44(2):283–292. doi: 10.1016/0092-8674(86)90762-2. [DOI] [PubMed] [Google Scholar]
  10. Kramer W., Fritz H. J. Oligonucleotide-directed construction of mutations via gapped duplex DNA. Methods Enzymol. 1987;154:350–367. doi: 10.1016/0076-6879(87)54084-8. [DOI] [PubMed] [Google Scholar]
  11. Kunze R., Stochaj U., Laufs J., Starlinger P. Transcription of transposable element Activator (Ac) of Zea mays L. EMBO J. 1987 Jun;6(6):1555–1563. doi: 10.1002/j.1460-2075.1987.tb02400.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lehto K., Dawson W. O. Changing the start codon context of the 30K gene of tobacco mosaic virus from "weak" to "strong" does not increase expression. Virology. 1990 Jan;174(1):169–176. doi: 10.1016/0042-6822(90)90065-y. [DOI] [PubMed] [Google Scholar]
  13. Lütcke H. A., Chow K. C., Mickel F. S., Moss K. A., Kern H. F., Scheele G. A. Selection of AUG initiation codons differs in plants and animals. EMBO J. 1987 Jan;6(1):43–48. doi: 10.1002/j.1460-2075.1987.tb04716.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McCLINTOCK B. Chromosome organization and genic expression. Cold Spring Harb Symp Quant Biol. 1951;16:13–47. doi: 10.1101/sqb.1951.016.01.004. [DOI] [PubMed] [Google Scholar]
  15. McClintock B. The significance of responses of the genome to challenge. Science. 1984 Nov 16;226(4676):792–801. doi: 10.1126/science.15739260. [DOI] [PubMed] [Google Scholar]
  16. Messing J. New M13 vectors for cloning. Methods Enzymol. 1983;101:20–78. doi: 10.1016/0076-6879(83)01005-8. [DOI] [PubMed] [Google Scholar]
  17. Mueller P. P., Hinnebusch A. G. Multiple upstream AUG codons mediate translational control of GCN4. Cell. 1986 Apr 25;45(2):201–207. doi: 10.1016/0092-8674(86)90384-3. [DOI] [PubMed] [Google Scholar]
  18. Ow D. W., DE Wet J. R., Helinski D. R., Howell S. H., Wood K. V., Deluca M. Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants. Science. 1986 Nov 14;234(4778):856–859. doi: 10.1126/science.234.4778.856. [DOI] [PubMed] [Google Scholar]
  19. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]

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