Table 1.
Effect of amino acid substitutions, small deletions and insertions in the Sco2149 cytoplasmic loop region on the ability of Sco2149TMD-AmiA and Sco2149TMD-Bla to support growth on SDS or ampicillin, respectively. Note that growth on ampicillin was scored using the tat- strain DADE and therefore assesses Sec translocation only. Y indicates growth on 1% SDS, N indicates no growth, nd – not determined. Mean M.I.C for growth on ampicillin is given in µg/ml + one standard deviation, n = at least 3. *Insertion of 3 additional amino acids, DEE between E128 and V129.
DOI: http://dx.doi.org/10.7554/eLife.26577.007
Table 1—source data 1. Images of SDS growth tests and M.I.C.Evaluator strip tests of all strain and plasmid combinations used in Table 1.
elife-26577-table1-data1.docx (6.7MB, docx)
DOI: 10.7554/eLife.26577.008
| Variant | Growth on 1% SDS (Tat translocation) |
Mean M.I.C. for ampicillin (Sec translocation) |
|---|---|---|
| wild type | Y | 7.6 ± 0.9 |
| R161K R162K | Y | 3.8 ± 0.5 |
| R161A R162D | N | 1.3 ± 0.3 |
| R161A R162A | N | 5.5 ± 1.0 |
| R161K R162Q | N | 3.3 ± 0.6 |
| ∆R161 ∆R162 | N | 9.3 ± 2.3 |
| R133H H134R | Y | 7.3 ± 1.2 |
| R133K H134K | Y | 7.0 ± 2.0 |
| M124L | Y | 6.0 ± 2.0 |
| M124A | Y | 6.0 ± 2.0 |
| S125L | Y | 6.7 ± 2.3 |
| S125A | Y | 8.0 ± 0.0 |
| D126L | Y | 7.3 ± 1.2 |
| D126A | Y | 7.3 ± 1.2 |
| E127L | Y | 6.0 ± 2.0 |
| E127A | Y | 8.0 ± 2.8 |
| A144P | Y | 6.7 ± 1.2 |
| A148P | Y | 8.0 ± 2.8 |
| A154P | Y | 8.0 ± 0.0 |
| ∆126–8 | nd | 8.0 ± 0.0 |
| ∆126–127 | nd | 7.5 ± 1.0 |
| ∆127–128 | nd | 8.0 ± 2.8 |
| 131–2 | nd | 7.3 ± 1.2 |
| ∆137∆141 | nd | 4.0 ± 0.0 |
| ∆131–2∆141 | nd | 6.0 ± 2.0 |
| Δ149Δ156 | nd | 8.0 ± 0.0 |
| ∆126–8 ∆137∆141 | nd | 8.0 ± 0.0 |
| ∆126–8∆131–2 | nd | 8.0 ± 2.4 |
| ∆126–8∆131–2 ∆137∆141 |
nd | 10.4 ± 2.2 |
| ∆126–8∆131–2 ∆137 ∆141 Δ149 Δ156 |
nd | 10.0 ± 2.8 |
| Ins D129 E130 E131* | nd | 6.7 ± 1.2 |
| D131A E132A | nd | 6.0 ± 2.3 |
| E137A E141A | nd | 6.7 ± 2.3 |
| D126A E127A E128A | nd | 7.2 ± 1.1 |
| D131K E132K | nd | 7.3 ± 1.2 |
| E137K E141K | nd | 6.5 ± 1.9 |
| D126K E127K E128K | nd | 9.0 ± 2.0 |
| D126K E127K E128K E137K E141K |
nd | 8.0 ± 0.0 |
| D126K E127K E128K D131K E132K E137K E141K |
nd | 10.0 ± 2.2 |