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. 2017 May 1;144(9):1648–1660. doi: 10.1242/dev.145607

Fig. 2.

Fig. 2.

Conditional mutation of Smc5 using the Zp3-Cre recombinase results in female infertility. (A) Schematic of mouse Smc5 floxed allele containing loxP sites, flanking exon 4 and the resulting Smc5-deleted allele after excision of exon 4 by Cre recombinase. Arrows represent primers for PCR genotyping of mice. (B) DNA agarose gel image of PCR products for genotyping. Lanes 1 and 2 represent a control genotype (Smc5 +/flox, Zp3-cre tg/0). Lane 1 (Smc5 +/flox): 410 bp wild-type allele, 563 bp and 644 bp flox allele. Lane 2 (Zp3-cre tg/0): 420 bp internal control and 281 bp Zp3-Cre transgene. Lanes 3 and 4 represent Smc5 cKO (Smc5 flox/del, Zp3-cre tg/0). Lane 3 (Smc5 flox/del): 563 bp and 644 bp flox allele, and 763 bp del allele. Lane 4 (Zp3-cre tg/0): 420 bp internal control and 281 bp Zp3-Cre transgene (same as lane 2).