Fig. 2. Requirement of S275, S276, S293, and T294 for maximal ZIP7 activation. Immunoblotting using pS473 AKT and pS275/S276 ZIP7 antibodies was performed in MCF-7 cells transfected with ZIP7 S275A, S276A, S293A, and T294A mutants and treated with zinc. Representative blots (A) and densitometric data for pS473 AKT (B) and pS275/S276 zip7 (C) are presented. Statistical significance is compared to time 0 of each construct. * = p < 0.05, ** = p < 0.01, and *** = p < 0.001.
