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. 1990 Aug;87(16):6398–6402. doi: 10.1073/pnas.87.16.6398

Differential cloning of genomic DNA: cloning of DNA with an altered primary structure by in-gel competitive reassociation.

H Yokota 1, M Oishi 1
PMCID: PMC54541  PMID: 2385598

Abstract

A procedure was developed for cloning (anonymous) DNA sequences whose primary structures differ between two DNA samples. The procedure is based upon in-gel competitive DNA reassociation after electrophoresis of a mixture of restriction enzyme-digested target DNA (from which clones are to be isolated) and a large excess of unclonable reference DNA (competitor DNA). Inclusion of polyethylene glycol in the reassociation buffer greatly improved the in-gel reassociation efficiency, which was critical for the practical use of the procedure. Using this technique, we obtained several clones from rat brain (target) DNA, which may have been derived from tissue (brain)-specific altered DNA structures. The details of this procedure and its possible applications are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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