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. 2017 May 22;9(5):124. doi: 10.3390/v9050124

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Neutralizing antibody response in piglets immunized with JEV G1 Laos or TRIP/JEV.prME. Groups of three pigs were either infected with JEV Laos or immunized with TRIP/JEV.prME or mock-inoculated, and serum was collected at the indicated time points (x-axis). (a) viral RNA load determined by RT-qPCR in sera from all nine animals; (b) JEV Laos- and (c) TRIP/JEV.prME-induced neutralizing antibody responses of sera against homologous JEV Laos (blue circles) and JEV Nakayama (red squares). Mean and standard deviations are shown. Statistical significance was determined after Log2 transformation of the data using two-way ANOVA and Sidak’s multiple comparison. * p < 0.05, ** p < 0.002, *** p < 0.001.

Neutralizing antibody response in piglets immunized with JEV G1 Laos or TRIP/JEV.prME. Groups of three pigs were either infected with JEV Laos or immunized with TRIP/JEV.prME or mock-inoculated, and serum was collected at the indicated time points (x-axis). (a) viral RNA load determined by RT-qPCR in sera from all nine animals; (b) JEV Laos- and (c) TRIP/JEV.prME-induced neutralizing antibody responses of sera against homologous JEV Laos (blue circles) and JEV Nakayama (red squares). Mean and standard deviations are shown. Statistical significance was determined after Log2 transformation of the data using two-way ANOVA and Sidak’s multiple comparison. * p < 0.05, ** p < 0.002, *** p < 0.001.