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. 2017 Jun 1;169(6):1078–1089.e13. doi: 10.1016/j.cell.2017.05.030

Figure 1.

Figure 1

The Cnn-CM2 Domain Is Required for Centrosomal Targeting and for Scaffold Assembly

(A) Micrographs illustrate and graphs quantify the mean centrosomal GFP-fluorescence levels in embryos of cnn mutant flies expressing GFP-Cnn or GFP-Cnn-ΔCM2. Inset shows a western blot probing the relative levels of GFP-Cnn or GFP-Cnn-ΔCM2 expressed in these embryos; actin is shown as a loading control.

(B) Micrographs illustrate the spontaneous assembly of cytoplasmic Cnn scaffolds in unfertilized eggs expressing GFP-Cnn-10D/E (15/15 injected eggs); no scaffolds were detectable in eggs expressing GFP-Cnn-10D/E-ΔCM2 (0/14 injected eggs). Error bars (A) represent the SD. Statistical significance was assessed using an unpaired t test in GraphPad Prism (∗∗∗∗p < 0.0001). Scale bars = 2 μm (A) and 5 μm (B).