Table 1. Oligonucleotide primers used in this study.
Gene | Forward primer | Reverse primer |
---|---|---|
Foxd1 | TCACTCCTCCCGCGCCCTTC | GCTCCGCTCCGCTACTTGGC |
Gdnf | GGTGTTGCTCCACACCGCGT | GGTGGCCGAGGGAGTGGTCT |
Pax2 | CCCAAGGTGGCAACGCCCAA | CGGCGTTGGGTGGAAAGGCT |
Wnt11 | TCCATCGAGCTCGCCCCCAA | TGGCCCCCATGAGGAGACCG |
Bmp4 | ACCACCTCAACTCAACCAATC | TCCACCACCATCTCCTGATAA |
Cdh11 | GCCGCCGCCGACTTGTGAAT | ATTTCTGGGGCCGTTGCGGG |
Calm1 | GAATGGCACCATTGACTTCC | GTAGCCATTGCCATCCTTGT |
Cfl1 | TTCTGGTAGGAGATGTGGGG | ACCAGGTCCTCCTTCTTGCT |
Ywhac | CCGGTATCTTGCTGAAGTCG | GGATCGGATGTGTAGGTTGC |
Acta | CGATAGAACACGGCATCATC | CATCAGGCAGTTCGTAGCTC |
Tgfb | TGAGTGGCTGTCTTTTGACG | TGGTTGTAGAGGGCAAGGAC |
Fn | GGGAAGAAAAGGAGCCCAGG | GGAAAAGTCCTGAGGTGGGG |
Cdkn1a | CGAGAACGGTGGAACTTTGAC | GAAATCTGTTAGGCTGGTCTGC |
Gpx1 | GGGCAAAGAAGATTCCAGGTT | AGAGCGGGTGAGCCTTCT |
Cat | AGGTGACACTATAGAATAGTG GTTTTCACCGACGAGAT |
GTACGACTCACTATAGGGACA CGAGGTCCCAGTTACCAT |
GR | TTCTGGAACTCGTCCACTAGG | CCATGTGGTTACTGCACTACTTCC |
Gst | GCCTTCTACCCGAAGACACCTT | GTCAGCCTGTTCCCTACA |
Gapdh | ACAGCAACAGGGTGGTGGAC | TTTGAGGGTGCAGCGAACTT |
Abbreviations: forkhead box D1 (Foxd1), glial cell derived neurotrophic factor (Gdnf), paired box 2 (Pax2) and wingless-type MMTV integration site family, member 11 (Wnt11), bone morphogenetic protein 4 (Bmp4), cadherin 11 (Cdh11), calmodulin 1 (Calm1), cofilin 1 (Cfl1), tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta (Ywhab), alpha-smooth muscle actin (Acta), transforming growth factor, beta (Tgfb), fibronectin (Fn), cyclin-dependent kinase inhibitor 1A (Cdkn1a), glutathione peroxidase 1 (Gpx1), catalase (Cat), glutathione reductase (GR) and glutathione-s-transferase (Gst).