Figure 2. HSP27 expressed in ALDH1^high cells derived from endometrial and ovarian carcinoma.

(A) HSP27 expression by qRT-PCR. HSP27 expression was assessed by real-time PCR using ALDH1^high and ALDH1^low cells derived from HEC-1, MCAS and HTBoA cells. Data represent means ± SD. *P values. (B) The expression of HSP27 in tumors derived from ALDHhigh cells and ALDHlow cell. Tumors derived from ALDH1^high cells and ALDH1^low cells in HEC-1 and MCAS cells were immunohistochemically stained by HSP27 antibody. Magnification ×200. (C) The expression of HSP27 in clinical samples. HSP27 protein expression was addressed using anti-HSP27 antibody. Total of 122 ovarian cancer cases were immunohistochemically stained. The expressions of HSP27 were evaluated as (score 0: < 15%, n = 40; score 1: 15%–30%, n = 41; score 2: > 30%, n = 41). (D) Overall survival. The differences of overall survival were examined for statistical significance using Fischer's test. *P values.