Abstract
The spread of wild-type Escherichia coli bearing a transferable plasmid was studied in a farm environment. E. coli of bovine and porcine origin were marked by resistance to nalidixic acid (Nar) or rifampicin (Rfr), and a transferable, multiple resistance plasmid (pSL222-1 derivative of plasmid R222) was introduced by conjugation. In separate experiments, the two mutant derivatives were fed back to the respective host animals, which were housed adjacent to, but separate from, one or more "recipient" animals. No antibiotic was given. Although the Rfr derivatives declined rapidly to undetectable levels within 1 week, the Nar mutants of bovine and porcine origin persisted in the original hosts and in their bedding throughout most of each 4-month test period. Test bacteria were isolated from mice residing in the same pen as the donor animals and from multiple secondary hosts having direct or indirect contact with the inoculated donors, but not from neighboring animals maintained in isolation. The bovine mutant was excreted by two caretakers for greater than 4 weeks and was recovered for 4-6 weeks from pigs, fowl, and flies. Although the porcine mutant appeared to colonize less effectively, it spread rapidly to flies and mice and was recovered transiently from humans and fowl. Despite high transfer rates of plasmid pSL222-1 from E. coli K-12 in vitro, transfer of the plasmid from the animal E. coli host was very low and transfer in vivo was not detected among indigenous gut or environmental bacteria. E. coli of animal origin can spread rapidly and can colonize the intestinal tract of humans and of other animals in the absence of antibiotic selection.
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Selected References
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- Anderson J. D., Gillespie W. A., Richmond M. H. Chemotherapy and antibiotic-resistance transfer between Enterobacteria in the human gastro-intestinal tract. J Med Microbiol. 1973 Nov;6(4):461–473. doi: 10.1099/00222615-6-4-461. [DOI] [PubMed] [Google Scholar]
- Compeau G., Al-Achi B. J., Platsouka E., Levy S. B. Survival of rifampin-resistant mutants of Pseudomonas fluorescens and Pseudomonas putida in soil systems. Appl Environ Microbiol. 1988 Oct;54(10):2432–2438. doi: 10.1128/aem.54.10.2432-2438.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cooke E. M., Hettiaratchy I. G., Buck A. C. Fate of ingested Escherichia coli in normal persons. J Med Microbiol. 1972 Aug;5(3):361–369. doi: 10.1099/00222615-5-3-361. [DOI] [PubMed] [Google Scholar]
- Dorn C. R., Tsutakawa R. K., Fein D., Burton G. C., Blenden D. C. Antibiotic resistance patterns of Escherichia coli isolated from farm families consuming home-raised meat. Am J Epidemiol. 1975 Oct;102(4):319–326. doi: 10.1093/oxfordjournals.aje.a112167. [DOI] [PubMed] [Google Scholar]
- Levy S. B., Marshall B., Schluederberg S., Rowse D., Davis J. High frequency of antimicrobial resistance in human fecal flora. Antimicrob Agents Chemother. 1988 Dec;32(12):1801–1806. doi: 10.1128/aac.32.12.1801. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Linton A. H., Howe K., Bennett P. M., Richmond M. H., Whiteside E. J. The colonization of the human gut by antibiotic resistant Escherichia coli from chickens. J Appl Bacteriol. 1977 Dec;43(3):465–469. doi: 10.1111/j.1365-2672.1977.tb00773.x. [DOI] [PubMed] [Google Scholar]
- Marshall B., Flynn P., Kamely D., Levy S. B. Survival of Escherichia coli with and without ColE1::Tn5 after aerosol dispersal in a laboratory and a farm environment. Appl Environ Microbiol. 1988 Jul;54(7):1776–1783. doi: 10.1128/aem.54.7.1776-1783.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Onderdonk A., Marshall B., Cisneros R., Levy S. B. Competition between congenic Escherichia coli K-12 strains in vivo. Infect Immun. 1981 Apr;32(1):74–79. doi: 10.1128/iai.32.1.74-79.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]