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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Traffic. 2017 Apr 4;18(5):315–329. doi: 10.1111/tra.12479

Table 1.

Yeast strains used in this study

Strain Genotype Source
BJ3505 MATa pep4::HIS3 prb1-Δ1.6R his3–200 lys2–801 trp1Δ101 (gal3) ura3–52 gal2 can1 90
DKY6281 MATα pho8::TRP1 leu2–3 leu 2–112 ura3–52 his3-Δ200 trp1-Δ901 lys2–801 82
RFY17 BJ3505, pah1Δ::kanMX6 19
RFY18 DKY6281, pahΔ::kanMX6 19
CHY53 BJ3505, yck3Δ::hphMX4 46
CHY54 DKY6281, yck3Δ::hphMX4 46
RFY60 BJ3505, dgk1Δ::hphMX4 This study
RFY61 DKY6281, dgk1Δ::hphMX4 This study
RFY62 RFY17, dgk1Δ:: hphMX4 This study
RFY63 RFY18, dgk1Δ:: hphMX4 This study
RFY64 RFY60, pRS416-DGK1 This study
RFY65 RFY61, pRS416-DGK1 This study
RFY66 RFY60, pRS416-DGK1D177A This study
RFY67 RFY61, pRS416-DGK1D177A This study
RFY68 BJ3505, DGK1::GFP-kanMX6 This study
RFY69 BJ3505, DGK1::3XHA-kanMX6 This study
RFY71 RFY68, pah1Δ:: hphMX4 This study
RFY72 RFY60, yck3Δ:: kanMX6 This study
RFY73 RFY61, yck3Δ:: kanMX6 This study