Table 1.

Sequence of oligonucleotides used for plasmid construction

Primer	Sequence
BipHcXbaI	5′-CTCTAGAGCATGAAGTTATGCATATTACTGGCCGTCGTGGCCTTTGTTGGCCTCTCGCTCGGGGATGTGCAGCTTCAGGA-3′
BipHcSacII	5′-TCCCCGCGGTTAGTGATGGTGATGGTGATGACTAGTACAATCCCTGG-3′
BipLcXbaI	5′-GC TCTAGA GCATGAAGTTATGCATATTACTGGCCGTCGTGGCCTTTGTTGGCCTCTCGCTCGGGGACATCAAGATGACCCAGTCT-3′
BipLcSacII	5′-TCCCCGCGGGGACTCGAGTGCGGCCGCACACTCATT-3′
CLR1for	5′-GTGTGCGGCCAGACTCGAGCACC-3′
CLR1rev	5′-GGTGCTCGAGTCTGGCCGCACAC-3′
CLR3rev	5′-TTCGAACCGCGGGGACTCGAGCCTACGTCTGGCCGCACACTC-3′
CLR5rev	5′-TTCGAACCGCGGGGACTCGAGGCGTCGCCTACGTCTGGCCGCAC-3′
pIHAneofor	5′-GACGGTATCGATAAGCTTGATAT-3′
CH1mycrev	5′-GAATTCCGCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCCTCGAGTGCGGCCGC-3′

Bold nucleotides encoding arginine, italic nucleotides encoding BiP signal sequence or Myc tag, underline restriction enzyme sites