Table 1. Quantitative analysis of ThT-monitored aggregation.
Aβ(M1-42) | [Aβ(M1-42)]DiY | [Aβ (M1-42)]Q-k | |||||||
---|---|---|---|---|---|---|---|---|---|
[Aβ] (μM) | 10 | 5 | 2.5 | 10 | 5 | 2.5 | 10 | 5 | 2.5 |
Lag Time (min) | 33 ± 6 | 43 ± 3 | 80 ± 5 | 33 ± 11 | 75 ± 7 | 180 ± 28 | 2 ± 3 | 13 ± 6 | 23 ± 6 |
Rate (RFU/min) | 185.5 ± 29.4 | 68.0 ± 18.3 | 11.0 ± 1.3 | 22.5 ± 0.4 | 15.4 ± 1.3 | 1.7 ± 1.3 | 136.0 ± 14.5 | 44.9 ±12.8 | 10.5 ± 2.2 |
Max Fluor. (RFU) | 10869 ± 990 | 9963 ± 363 | 2618 ± 90 | 18660 ± 990 | 9963 ± 343 | 4400 ± 226 | 11110 ± 1512 | 4810 ± 1463 | 1733 ± 339 |
t = max | ~ 5 hrs | - | - | ~ 17 hrs | - | - | ~ 5.5 hrs | - | - |
t = ½ max | ~ 1 hr | - | - | ~ 6.5 hrs | - | - | ~1 hr | - | - |
t = ¼ max | ~ 30 min | - | - | ~ 3 hrs | - | - | ~ 30 min | - | - |
Time points were calculated based on criteria described in Supplementary Figure 4 and are the averaged from at least two separate experiments.
Values are mean ± standard deviation are given.