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GBM28 and SJ-GBM2 cells were treated with penfluridol (5 μM) for 48 h after (A–B) cells were pretreated with 30 μM GANT61 for 3 h (C–D) cells were pretreated with 25 μM Cyclopamine for 3 h (E–G) GBM28, SJ-GBM2 and U87MG cells were transfected with GLI1 siRNA. Actin was used as a loading control. Figures shown are the representatives blots of three independent experiments.
