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. 2017 Apr 4;8(20):33571–33585. doi: 10.18632/oncotarget.16827

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Copyright: © 2017 Shlamkovich et al.

This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.

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(A) TIME cells were treated with control buffer (basal level, black), 200 ng/ml of Ang1 (green), 200 ng/ml of Ang1 and 1 μM Ang2-BD_WT (brown), 1 μM Ang2-BD_WT(red), 200 ng/ml of Ang1 and 1 μM Ang2-BD_c2.36 (blue) and 1 μM Ang2-BD_C2.36 (purple) for 15 min for Tie2 phosphorylation. (B) Cell lysates were analyzed by western blot using antibodies against pTie2, Tie2 and β-actin. # indicates P value < 0.05 for comparison of results between Ang2-BD_WT and Ang2-BD_C2.36; ## indicates P value < 0.01 for comparison of results between Ang1 + Ang2-BD_WT and Ang2-BD_WT and between Ang1 + Ang2-BD_C2.36 and Ang2-BD_C2.36; *indicates P value < 0.05 for comparison of results between Ang1 and Ang1 + Ang2-BD_WT; **indicates P value < 0.01 for comparison of results between Ang1 and Ang1 + Ang2-BD_C2.36. Data shown is the average of triplicate experiments, and error bars represent standard error of the mean.