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. 2017 Apr 4;8(20):33779–33795. doi: 10.18632/oncotarget.16810

Figure 3. SEL120-34A inhibits interferon- induced genes expression in a dose dependent manner.

Figure 3

(A) HCT-116 and Colo-205 cells were made quiescent overnight by decreasing FBS in medium to 0.5% and then preincubated with 0.5 μM SEL120-34A for 1 h followed by either IFNg (5 ng/mL) or IFNa (250 units/mL) stimulation in the presence of SEL120-34A. At the indicated time point, cells were harvested, RNA extracted and transcripts levels analyzed by the qRT-PCR reaction. The results were normalized for RPLP0 mRNA (means ±S.D., n=3). (B) Colo-205 cells were synchronized as in panel A and then challenged with increasing doses of SEL120-34A for 1 h followed by either 3 h IFNg (5 ng/μl) or 48 h IFNa (250 units/ml) stimulation. At this time RNA was extracted, DNAse I digested and subjected to the qRT-PCR reaction. The results were normalized for RPLP0 mRNA (means ±S.D., n=3). Differences between mRNA levels for control and SEL120-34A at the indicated time points were assessed by t-tests. A p value of ≤ 0.05 (*) was considered significant.