Abstract
A new liquid medium, called “dulcitol selenite enrichment,” has been developed for the detection and enumeration of Salmonella in foods. The medium is not only highly selective in enriching Salmonella and inhibiting completely or appreciably other extraneous organisms commonly found in seafoods, but is also highly sensitive in recovering as low as 2 to 7 cells of Salmonella, even in the presence of large numbers (104 to 106 cells) of mixed flora common to these foods. The addition of seafood material does not seem to interfere with the sensitivity, selectivity, or productivity of the medium. Even physiologically debilitated cells of Salmonella were enriched well enough in this medium to be detected easily.
Full text
PDF








Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BANWART G. J., AYRES J. C. Effect of various enrichment broths and selective agars upon the growth of several species of Salmonella. Appl Microbiol. 1953 Nov;1(6):296–301. doi: 10.1128/am.1.6.296-301.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HAJNA A. A., DAMON S. R. New enrichment and plating media for the isolation of Salmonella and Shigella organisms. Appl Microbiol. 1956 Nov;4(6):341–345. doi: 10.1128/am.4.6.341-345.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HARVEY R. W., PHILLIPS W. P. An environmental survey of bakehouses and abattoirs for salmonellae. J Hyg (Lond) 1961 Mar;59:93–103. doi: 10.1017/s0022172400038730. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HURLEY N. A., AYRES J. C. A comparison of six enrichment media for isolating Salmonella pullorum from egg-products. Appl Microbiol. 1953 Nov;1(6):302–306. doi: 10.1128/am.1.6.302-306.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KONFORTI N., NAVON B., RAPPAPORT F. A new enrichment medium for certain Salmonellae. J Clin Pathol. 1956 Aug;9(3):261–266. doi: 10.1136/jcp.9.3.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Levine V. E. THE REDUCING PROPERTIES OF MICROORGANISMS WITH SPECIAL REFERENCE TO SELENIUM COMPOUNDS. J Bacteriol. 1925 May;10(3):217–263. doi: 10.1128/jb.10.3.217-263.1925. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NORTH W. R., BARTRAM M. T. The efficiency of selenite broth of different compositions in the isolation of Salmonella. Appl Microbiol. 1953 May;1(3):130–134. doi: 10.1128/am.1.3.130-134.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NORTH W. R., Jr Lactose pre-enrichment method for isolation of Samonella from dried egg albumen. Its use in a survery of commercially produced albumen. Appl Microbiol. 1961 May;9:188–195. doi: 10.1128/am.9.3.188-195.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SILLIKER J. H., DEIBEL R. H., FAGAN P. T. ISOLATION OF SALMONELLAE FROM FOOD SAMPLES. VI. COMPARISON OF METHODS FOR THE ISOLATION OF SALMONELLA FROM EGG PRODUCTS. Appl Microbiol. 1964 May;12:224–228. doi: 10.1128/am.12.3.224-228.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SILLIKER J. H., TAYLOR W. I. Isolation of salmonellae from food samples. II. The effect of added food samples upon the performance of enrichment broths. Appl Microbiol. 1958 Jul;6(4):228–232. doi: 10.1128/am.6.4.228-232.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SMITH H. G. On the nature of the selective action of selenite broth. J Gen Microbiol. 1959 Aug;21:61–71. doi: 10.1099/00221287-21-1-61. [DOI] [PubMed] [Google Scholar]
- STOKES J. L., OSBORNE W. W. A selenite brilliant green medium for the isolation of Salmonella. Appl Microbiol. 1955 Jul;3(4):217–220. doi: 10.1128/am.3.4.217-220.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TAYLOR W. I. Isolation of Salmonellae from food samples. V. Determination of the method of choice for enumeration of Salmonella. Appl Microbiol. 1961 Nov;9:487–490. doi: 10.1128/am.9.6.487-490.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TAYLOR W. I., SILLIKER J. H., ANDREWS H. P. Isolation of Salmonellae from food samples. I. Factors affecting the choice of media for the detection and enumeration of Salmonella. Appl Microbiol. 1958 May;6(3):189–193. doi: 10.1128/am.6.3.189-193.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TAYLOR W. I., SILLIKER J. H. Isolation of Salmonellae from food samples. IV. Comparison of methods of enrichment. Appl Microbiol. 1961 Nov;9:484–486. doi: 10.1128/am.9.6.484-486.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]