Abstract
Escherichia coli cultures grown on Tergitol-7 medium, with 2,3,5-triphenyltetrazolium chloride added, produced three main types of colonies: rough, intermediate, and mucoid. These colonies were yellow to amber in color and produced slight yellow zones in the medium. Rough colonies were flat, dry, and spreading, with a cut-glass appearance. Intermediate-type colonies varied considerably, but could be divided into two general subtypes. Intermediate-rough colonies had the cut-glass appearance characteristic of rough colonies, but were much more compact and raised, with irregular edges. Intermediate-smooth colonies had a slight cut-glass appearance, but were smooth and entire. Mucoid-type colonies also appeared in two subtypes. Mucoid A colonies were mucoid hemispheres. Mucoid B colonies, after incubation at 37 C for 24 hr, appeared as small, intermediate colonies. However, during a 24-hr holding period at room temperature, mucuslike material proliferated around the colonies. A fourth type of colony was red with blue surrounding medium. Only mucoid-type cultures could not be serologically O-grouped.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CHAPMAN G. H. A culture medium for detecting and confirming Escherichia coli in ten hours. Am J Public Health Nations Health. 1951 Nov;41(11 Pt 1):1381–1381. doi: 10.2105/ajph.41.11_pt_1.1381. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pollard A. L. A Useful Selective Bactericidal Property of Tergitol-7. Science. 1946 Jun 28;103(2687):758–759. doi: 10.1126/science.103.2687.758. [DOI] [PubMed] [Google Scholar]
- WISEMAN R. F., SARLES W. B. A plating technique for screening intestinal coliform bacteria. J Bacteriol. 1956 Apr;71(4):480–481. doi: 10.1128/jb.71.4.480-481.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]