Figure 8. Host-adapted binK1 attenuates quorum-sensing regulation of luminescence.

(A) Supernatant concentrations (nM/OD₆₀₀) of N-(3-oxohexanoyl) homoserine lactone (C6-HSL), as quantified against synthetic standards (Schaefer et al., 2000; Pearson et al., 1994; Duerkop et al., 2007) and corresponding luminescence (Lum/OD₆₀₀) of 10 independent cultures each for wild-type MJ11, binK1 and ΔbinK derivatives during quorum-sensing induction of luminescence determined from cultures grown to early log (Average OD₆₀₀ 1.1, range 0.9–1.4,). (B) Average cell density as measured by absorbance (OD₆₀₀), colony-forming units (CFU)/mL/OD₆₀₀, N-(3-oxohexanoyl) homoserine lactone (C6) nM concentration, N-octanoyl homoserine lactone (C8) nM concentration, and luminescence (Lum)/1 mL culture for ten biological replicates of each variant relative to wild-type MJ11. Error bars: 95% CI. Significant p-values (p<0.05) are indicated above each comparison. *p<2.2e-16.

DOI: http://dx.doi.org/10.7554/eLife.24414.020