Abstract
Pseudomonas C12B grew on and oxidized linear primary alcohols with even- and odd-numbered carbon chains ranging from C2 to C11. Cell-free extracts of the bacteria contained a nicotinamide adenine dinucleotide-linked dehydrogenase(s) active with these alcohols and with branched primary and linear secondary alcohols as well. Analysis by gas-liquid chromatography of hexane extracts of filtrates of cultures containing mixtures of even-carbon numbered alcohols from C10 to C18 revealed that decanol was rapidly utilized, whereas the remainder were slowly dissimilated up to 19 hr and then were rapidly degraded in the next few hours of culture. The validity for these studies of (i) steam distillation as a method for collecting the alcohols from cultures, and (ii) quantitative estimation by gas-liquid chromatographic comparison with an added internal marker, was established. Steam distillation and gas-liquid chromatography were then used to show that failure to demonstrate stoichiometry of sulfate and dodecanol in the alkyl sulfatase reaction in a previous study resulted from contamination of the commercial “Dodecyl Sodium Sulfate, 95%” used with decyl, undecyl, and tetradecyl sulfates.
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Selected References
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