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. 1966 Mar;14(2):223–228. doi: 10.1128/am.14.2.223-228.1966

Use of the Anaerobic Pouch in Isolating Clostridium botulinum Spores from Fresh Meats

Richard A Greenberg 1, Bendt O Bladel 1, Walter J Zingelmann 1
PMCID: PMC546654  PMID: 5335387

Abstract

The anaerobic film pouch was demonstrated to be an effective device for the primary isolation of Clostridium botulinum types A and B spores from raw pork, beef, and chicken. Optimal pasteurization of these meats (for reduction of nonspore microflora without affecting indigenous putrefactive anaerobic spore levels) was 50 min at 60 C. C. botulinum spores were recovered with good precision from meat samples inoculated with mixtures of C. botulinum and Putrefactive Anaerobe 3679 at 1:1 and at 1:99 ratios. Verification of C. botulinum isolates was accomplished by protection testing of subcultures in mice.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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