Abstract
A simple, rapid method, based on the Oudin single diffusion technique, is described for the quantitative assay of staphylococcal enterotoxin B. The method yields reproducible results without close control of such assay variables as temperature, antiserum dilution, and assay time, provided that the ionic strength is maintained above 0.2 n sodium chloride equivalent.
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Selected References
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- HALL H. E., ANGELOTTI R., LEWIS K. H. QUANTITATIVE DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN B IN FOOD BY GEL-DIFFUSION METHODS. Public Health Rep. 1963 Dec;78:1089–1098. [PMC free article] [PubMed] [Google Scholar]
- OUDIN J. B. Specific precipitation in gels and its application to immunochemical analysis. Methods Med Res. 1952;5:335–378. [PubMed] [Google Scholar]
- RUBINSTEIN H. M. Quantitative antigen analysis by the Oudin method. J Immunol. 1954 Nov;73(5):322–330. [PubMed] [Google Scholar]
- SILVERMAN S. J. SEROLOGICAL ASSAY OF CULTURE FILTRATES FOR STAPHYLOCOCCUS ENTEROTOXIN. J Bacteriol. 1963 Apr;85:955–956. doi: 10.1128/jb.85.4.955-956.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schantz E. J., Roessler W. G., Wagman J., Spero L., Dunnery D. A., Bergdoll M. S. Purification of staphylococcal enterotoxin B. Biochemistry. 1965 Jun;4(6):1011–1016. doi: 10.1021/bi00882a005. [DOI] [PubMed] [Google Scholar]