Figure 6.

Depletion of Gr-1+CD11b+ myeloid cells induces severe disease in DSS-induced colitis. (A) WT mice were treated with anti-Gr-1 or isotype control mAb. Representative dot plots show that anti-Gr-1 effectively depleted this subset from LPL, mLN and periphery. (B) Mice injected with Gr-1 or isotype mAb were fed with DSS water for 7 d. Representative histological slides show less inflammatory cell infiltration in Gr-1 depleted mice. Summarized inflammatory cell infiltration scores are shown. (C) Representative histological slides show severe mucosal erosion and ulceration in Gr-1 depleted mice. Summarized ulceration scores are shown. *p < 0.05.