Skip to main content
NCBI home page
Applied Microbiology logoLink to Applied Microbiology
. 1967 Jan;15(1):31–34. doi: 10.1128/am.15.1.31-34.1967

Antibiotic Activity in the Presence of Agar

F J Hanus 1, J G Sands 1, E O Bennett 1
PMCID: PMC546838  PMID: 4291669

Abstract

Agar has been shown to interfere with the activity of some antibiotics against Staphylococcus aureus. This interference has been observed as an increase in the minimal inhibitory concentration and in the diameter of the zone of inhibition. Purifying the agar with water extractions substantially reduced this adverse effect.

Full text

PDF
31

Images in this article

32Fig. 1
on p.32

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRISHAMMAR S., HJERTEN S., von HOFSTEN Immunological precipitates in agarose gels. Biochim Biophys Acta. 1961 Nov 11;53:518–521. doi: 10.1016/0006-3002(61)90211-6. [DOI] [PubMed] [Google Scholar]
  2. CAMPBELL J. B., COLTER J. S. STUDIES OF THREE VARIANTS OF MENGO ENCEPHALOMYELITIS VIRUS. 3. EFFECT OF OVERLAY AND POLYANIONS OF PLAQUE SIZE. Virology. 1965 Apr;25:608–619. doi: 10.1016/0042-6822(65)90089-9. [DOI] [PubMed] [Google Scholar]
  3. COLTER J. S., DAVIES M. A., CAMPBELL J. B. STUDIES OF THREE VARIANTS OF MENGO ENCEPHALOMYELITIS VIRUS. II. INHIBITION OF INTERACTION WITH L CELLS BY AN AGAR INHIBITOR AND BY PROTAMINE. Virology. 1964 Dec;24:578–585. doi: 10.1016/0042-6822(64)90210-7. [DOI] [PubMed] [Google Scholar]
  4. Donovick R., Bayan A. P., Canales P., Pansy F. The Influence of Certain Substances on the Activity of Streptomycin: III. Differential Effects of Various Electrolytes on the Action of Streptomycin. J Bacteriol. 1948 Jul;56(1):125–137. [PMC free article] [PubMed] [Google Scholar]
  5. EISENBERG G. M., FLIPPIN H. F., WEISS W. Microbiologic studies and preliminary clinical experiences with nystatin. I. In vitro susceptibility of Candida albicans. Antibiot Annu. 1955;3:837–841. [PubMed] [Google Scholar]
  6. GREENBERG J. A factor in agar which reverses the antibacterial activity of 1-methyl-3-nitrosoguanidine. Nature. 1960 Nov 19;188:660–660. doi: 10.1038/188660a0. [DOI] [PubMed] [Google Scholar]
  7. HANUS F. J., BENNETT E. O. THE EFFECT OF AGAR ON THE INHIBITORY ACTIVITIES OF FATTY AMINES. Antonie Van Leeuwenhoek. 1964;30:412–416. doi: 10.1007/BF02046754. [DOI] [PubMed] [Google Scholar]
  8. HJERTEN S. A new method for preparation of agaraose for gel electrophoresis. Biochim Biophys Acta. 1962 Aug 27;62:445–449. doi: 10.1016/0006-3002(62)90224-x. [DOI] [PubMed] [Google Scholar]
  9. HJERTEN S. Agarose as an anticonvection agent in zone electrophoresis. Biochim Biophys Acta. 1961 Nov 11;53:514–517. doi: 10.1016/0006-3002(61)90210-4. [DOI] [PubMed] [Google Scholar]
  10. IYER R., IYER V. Effect of agar on the inhibition of Micrococcus pyogenes var. aureus by chlortetracycline and other antibiotics. Antibiot Chemother (Northfield) 1960 Jul;10:409–413. [PubMed] [Google Scholar]
  11. JACKSON G. G., FINLAND M. Comparison of methods for determining sensitivity of bacteria to antibiotics in vitro. AMA Arch Intern Med. 1951 Oct;88(4):446–460. doi: 10.1001/archinte.1951.03810100030003. [DOI] [PubMed] [Google Scholar]
  12. MCCORMICK M. H., MCGUIRE J. M., PITTENGER G. E., PITTENGER R. C., STARK W. M. Vancomycin, a new antibiotic. I. Chemical and biologic properties. Antibiot Annu. 1955;3:606–611. [PubMed] [Google Scholar]
  13. SANDS J. G., GOERS L. A., BENNETT E. O. THE EFFECT OF AGAR ON THE INHIBITORY ACTIVITY OF PHENOLS. Antonie Van Leeuwenhoek. 1963;29:386–392. doi: 10.1007/BF02046091. [DOI] [PubMed] [Google Scholar]
  14. SCHULZE I. T., SCHLESINGER R. W. Inhibition of infectious and hemagglutinating properties of type 2 dengue virus by aqueous Agar extracts. Virology. 1963 Jan;19:49–57. doi: 10.1016/0042-6822(63)90023-0. [DOI] [PubMed] [Google Scholar]
  15. SPAULDING E. H., ANDERSON T. G. Selection of antimicrobial agents by laboratory means. J Am Med Assoc. 1951 Dec 1;147(14):1336–1341. doi: 10.1001/jama.1951.03670310026010. [DOI] [PubMed] [Google Scholar]
  16. TAKEMOTO K. K., LIEBHABER H. Virus-polysaccharide interactions. I. An agar polysaccharide determining plaque morphology of EMC virus. Virology. 1961 Aug;14:456–462. doi: 10.1016/0042-6822(61)90338-5. [DOI] [PubMed] [Google Scholar]
  17. WEINBERG E. D. The mutual effects of antimicrobial compounds and metallic cations. Bacteriol Rev. 1957 Mar;21(1):46–68. doi: 10.1128/br.21.1.46-68.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES