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. 1967 Jan;15(1):117–119. doi: 10.1128/am.15.1.117-119.1967

Isolation of Thermophiles from Broadleaf Tobacco and Effect of Pure Culture Inoculation on Cigar Aroma and Mildness

Clyde F English 1, Emily J Bell 1, Alfred J Berger 1,1
PMCID: PMC546854  PMID: 16349710

Abstract

Thermophilic members of the genus Bacillus isolated from fermented Connecticut broadleaf tobacco included eight strains of B. subtilis, five strains of B. coagulans, four strains of B. megaterium, and three strains of B. circulans. Some of these strains in pure single or mixed culture were employed to enrich the normal thermophile flora of “sweating” tobacco. Three strains of B. subtilis and one of B. circulans, either in single or multiple enrichment, caused the more rapid appearance of a pleasing aroma in Pennsylvania “Wrapper B” filler tobacco. These conclusions are based on subjective reactions of professional testers after numerous blindfold smoking tests.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. KNIGHT B. C. J. G., PROOM H. A comparative survey of the nutrition and physiology of mesophilic species in the genus Bacillus. J Gen Microbiol. 1950 Sep;4(3):508–538. doi: 10.1099/00221287-4-3-508. [DOI] [PubMed] [Google Scholar]

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