Abstract
Methods using dialysis or ultrafiltration are described for the collection of extracellular fluid in rumen contents for analysis of amino acids. Marked differences in the concentration of aspartic acid, glutamic acid, and alanine were found in samples of either diffusate or ultrafiltrate and in clarified acidified rumen liquor. Concentrations are given for aspartic acid, glutamic acid, alanine, glycine, γ-aminobutyric acid, valine, δ-aminovaleric acid, and leucine.
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- ANNISON E. F. Nitrogen metabolism in the sheep; protein digestion in the rumen. Biochem J. 1956 Dec;64(4):705–714. doi: 10.1042/bj0640705. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ATFIELD G. N., MORRIS C. J. Analytical separations by highvoltage paper electrophoresis. Amino acids in protein hydrolysates. Biochem J. 1961 Dec;81:606–614. doi: 10.1042/bj0810606. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CHALMERS M. I., SYNGE R. L. The digestion of protein and nitrogenous compounds in ruminants. Adv Protein Chem. 1954;9:93–120. doi: 10.1016/s0065-3233(08)60205-3. [DOI] [PubMed] [Google Scholar]
- EL-SHAZLY K. Degradation of protein in the rumen of the sheep. II. The action of rumen micro-organisms on amino acids. Biochem J. 1952 Aug;51(5):647–653. doi: 10.1042/bj0510647. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEWIS D. Amino-acid metabolism in the rumen of the sheep. Br J Nutr. 1955;9(3):215–230. doi: 10.1079/bjn19550035. [DOI] [PubMed] [Google Scholar]
- MATHESON N. A. An improved method of separating amino acids as N-2,4-dinitrophenyl derivatives. Biochem J. 1963 Jul;88:146–151. doi: 10.1042/bj0880146. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MATHESON N. A. The quantitative isolation of valine from amino acid mixtures as its dinitrophenyl derivative. Biochem J. 1963 Jul;88:152–155. doi: 10.1042/bj0880152. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PORTUGAL A. V., GREEN R., SUTHERLAND T. M. SEPARATION AND ESTIMATION OF THE 2,4-DINITROPHENYL DERIVATIVES OF GLUTAMIC AND ASPARTIC ACIDS. J Chromatogr. 1963 Oct;12:183–188. doi: 10.1016/s0021-9673(01)83668-2. [DOI] [PubMed] [Google Scholar]
- Smith P. H., Mah R. A. Kinetics of acetate metabolism during sludge digestion. Appl Microbiol. 1966 May;14(3):368–371. doi: 10.1128/am.14.3.368-371.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]