Abstract
Foot-and-mouth disease virus can be grown in baby hamster kidney cells with a chemically characterized medium containing only tris(hydroxymethyl)-amino-methane (Tris) buffer, glucose, glutamine, and salts. Virus infectivity was only 0.5 log unit less than in a complex cell growth medium containing serum, tryptose phosphate, and lactalbumin hydrolysate. At high multiplicity of infection, production was maximal in 5 hr, with the virus remaining largely intracellular. Glucose and glutamine appeared to act independently of each other although both were required at about the same time during the virus production cycle. Glutamine had the greater effect and could not be replaced by amino acids, purines, and pyrimidines. Glutamine also stimulated cellular oxygen uptake in both normal and infected cells. Serum and other organic components added singly to the defined medium did not increase the virus yield. Studies on uninfected cells over a 5-hr incubation period showed that the defined medium maintained protein and ribonucleic acid synthesis at rates similar to the complex cell growth medium. These rates were much lower in media containing only inorganic salts and Tris buffer. Glucose, however, was more important to uninfected cellular metabolism than was glutamine. Defined medium containing dialyzed calf serum produced the highest rate of protein synthesis.
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- BACHRACH H. L., CALLIS J. J., HESS W. R., PATTY R. E. A plaque assay for foot-and-mouth disease virus and kinetics of virus reproduction. Virology. 1957 Oct;4(2):224–236. doi: 10.1016/0042-6822(57)90060-0. [DOI] [PubMed] [Google Scholar]
- Crabtree H. G. Observations on the carbohydrate metabolism of tumours. Biochem J. 1929;23(3):536–545. doi: 10.1042/bj0230536. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EAGLE H., HABEL K. The nutritional requirements for the propagation of poliomyelitis virus by the HeLa cell. J Exp Med. 1956 Aug 1;104(2):271–287. doi: 10.1084/jem.104.2.271. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PLEDGER R. A., POLATNICK J. Defined medium for growth of foot-and-mouth disease virus. J Bacteriol. 1962 Mar;83:579–583. doi: 10.1128/jb.83.3.579-583.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- POLATNICK J., BACHRACH H. L. Metabolic studies of bovine kidney cultures infected with foot-and-mouth disease virus. Virology. 1960 Nov;12:450–462. doi: 10.1016/0042-6822(60)90166-5. [DOI] [PubMed] [Google Scholar]
- POLATNICK J., BACHRACH H. L. PRODUCTION AND PURIFICATION OF MILLIGRAM AMOUNTS OF FOOT-AND-MOUTH DISEASE VIRUS FROM BABY HAMSTER KIDNEY CELL CULTURES. Appl Microbiol. 1964 Jul;12:368–373. doi: 10.1128/am.12.4.368-373.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]