Skip to main content
Applied Microbiology logoLink to Applied Microbiology
. 1967 Jul;15(4):785–789. doi: 10.1128/am.15.4.785-789.1967

Effects of Continuous and Interrupted Radiation on Microorganisms

P M Borick 1, M G Fogarty 1
PMCID: PMC547064  PMID: 4963440

Abstract

Various bacterial spores exhibited a wide range of radiation resistance to doses of 0.25 to 2.5 Mrad from a cobalt-60 radiation facility. Bacillus pumilus and Clos-tridium tetani were shown to have the highest degree of resistance when compared with other bacterial sporeformers. B. subtilis E163 was the least resistant of the bacterial spores studied. Dried spores contained on cellulose discs were more readily destroyed by γ-rays than were wet spores under similar conditions. Mycobacterium tuberculosis was destroyed by radiation doses much lower than that required by the least resistant bacterial spores. Interrupted dosimetry tests performed with materials of various types showed that sutures and other similar materials were effectively sterilized when the total radiation dose was given in two separate exposures with periods of interruption of 1 to 19 days. When “agar dosimeters” were employed in similar interrupted dosimetry series, B. pumilus spores were recovered in a few tests after administration of a total combined dosage of 2.5 Mrad with interruption periods of 2 to 19 days. When the experiment was repeated with interruption for 14 days, no survivors were found after a total dose of 2.0 to 2.8 Mrad.

Full text

PDF
785

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALPER T., GILLIES N. E., ELKIND M. M. The sigmoid survival curve in radiobiology. Nature. 1960 Jun 25;186:1062–1063. doi: 10.1038/1861062a0. [DOI] [PubMed] [Google Scholar]
  2. BRIDGES A. E., OLIVO J. P., CHANDLER V. L. Relative resistances of micro-organisms to cathode rays. II. Yeasts and molds. Appl Microbiol. 1956 May;4(3):147–149. doi: 10.1128/am.4.3.147-149.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DUGGAN D. E., ANDERSON A. W., ELLIKER P. R. INACTIVATION OF THE RADIATION-RESISTANT SPOILAGE BACTERIUM MICROCOCCUS RADIODURANS. I. RADIATION INACTIVATION RATES IN THREE MEAT SUBSTRATES AND IN BUFFER. Appl Microbiol. 1963 Sep;11:398–403. doi: 10.1128/am.11.5.398-403.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. KOH W. Y., MOREHOUSE C. T., CHANDLER V. L. Relative resistances of micro-organisms to cathode rays. I. Nonsporeforming bacteria. Appl Microbiol. 1956 May;4(3):143–146. doi: 10.1128/am.4.3.143-146.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. PEPPER R. E., BUFFA N. T., CHANDLER V. L. Relative resistances of micro-organisms to cathode rays. III. Bacterial spores. Appl Microbiol. 1956 May;4(3):149–152. doi: 10.1128/am.4.3.149-152.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES