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. 1967 Jul;15(4):815–818. doi: 10.1128/am.15.4.815-818.1967

Enterotoxin B: Serological Assay in Cultures by Passive Hemagglutination

H M Johnson 1, H E Hall 1, M Simon 1
PMCID: PMC547071  PMID: 4963442

Abstract

Bis-diazotized benzidine hemagglutination with Formalinized sheep erythrocytes was adapted to the rapid and specific detection of enterotoxin B in staphylococcal culture fluids. There was complete agreement between hemagglutination inhibition (HI) and gel diffusion in detecting 8 enterotoxin B-positive cultures from a total of 68 staphylococcal cultures tested. The sensitivity of HI equals or exceeds that of gel diffusion. Also, results can be obtained in several hours, even with extremely low concentrations of enterotoxin, whereas it may require 24 hr to 1 week to obtain comparable results with gel diffusion. Problems associated with the presence of potent hemagglutinins for sheep erythrocytes in several staphylococcal culture fluids are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BUTLER W. T. HEMAGGLUTINATION STUDIES WITH FORMALINIZED ERYTHROCYTES. EFFECT OF BIS-DIAZO-BENZIDINE AND TANNIC ACID TREATMENT ON SENSITIZATION BY SOLUBLE ANTIGEN. J Immunol. 1963 May;90:663–671. [PubMed] [Google Scholar]
  2. Bergdoll M. S., Chu F. S., Huang I. Y., Rowe C., Shih T. Staphylococcal enterotoxin B. 3. The physicochemical properties and the N- and C-terminal amino acid sequences. Arch Biochem Biophys. 1965 Oct;112(1):104–110. doi: 10.1016/0003-9861(65)90016-0. [DOI] [PubMed] [Google Scholar]
  3. CASMAN E. P., BENNETT R. W. DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN IN FOOD. Appl Microbiol. 1965 Mar;13:181–189. doi: 10.1128/am.13.2.181-189.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CROWLE A. J. A simplified micro double-diffusion agar precipitin technique. J Lab Clin Med. 1958 Nov;52(5):784–787. [PubMed] [Google Scholar]
  5. FRIEDMAN M. E., WHITE J. D. IMMUNOFLUORESCENT DEMONSTRATION OF CELL-ASSOCIATED STAPHYLOCOCCAL ENTEROTOXIN B. J Bacteriol. 1965 Apr;89:1155–1155. doi: 10.1128/jb.89.4.1155-1155.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HALL H. E., ANGELOTTI R., LEWIS K. H. DETECTION OF THE STAPHYLOCOCCAL ENTEROTOXINS IN FOOD. Health Lab Sci. 1965 Jul;2:179–191. [PubMed] [Google Scholar]
  7. HALL H. E., ANGELOTTI R., LEWIS K. H. QUANTITATIVE DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN B IN FOOD BY GEL-DIFFUSION METHODS. Public Health Rep. 1963 Dec;78:1089–1098. [PMC free article] [PubMed] [Google Scholar]
  8. Johnson H. M., Brenner K., Angelotti R., Hall H. E. Serological studies of types A, B, and E botulinal toxins by passive hemagglutination and bentonite flocculation. J Bacteriol. 1966 Mar;91(3):967–974. doi: 10.1128/jb.91.3.967-974.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. SILVERMAN S. J. SEROLOGICAL ASSAY OF CULTURE FILTRATES FOR STAPHYLOCOCCUS ENTEROTOXIN. J Bacteriol. 1963 Apr;85:955–956. doi: 10.1128/jb.85.4.955-956.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schantz E. J., Roessler W. G., Wagman J., Spero L., Dunnery D. A., Bergdoll M. S. Purification of staphylococcal enterotoxin B. Biochemistry. 1965 Jun;4(6):1011–1016. doi: 10.1021/bi00882a005. [DOI] [PubMed] [Google Scholar]
  11. Weirether F. J., Lewis E. E., Rosenwald A. J., Lincoln R. E. Rapid quantitative serological assay of staphylococcal enterotoxin B. Appl Microbiol. 1966 Mar;14(2):284–291. doi: 10.1128/am.14.2.284-291.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]

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