Abstract
The aminopeptidase specificity of 24 strains of bacteria was determined fluorometrically by use of a series of α-amino acid β-naphthylamides as substrates. Provided that strict control over medium and growth time was adhered to, a reproducible profile of aminopeptidase activity was obtained which could be used for the identification of bacteria.
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Selected References
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- GOLDSTEIN T. P., PLAPINGER R. E., NACHLAS M. M., SELIGMAN A. M. SYNTHESIS OF CHROMOGENIC SUBSTRATES FOR THE ASSAY OF AMINOPEPTIDASE ACTIVITY. J Med Pharm Chem. 1962 Jul;91:852–857. doi: 10.1021/jm01239a020. [DOI] [PubMed] [Google Scholar]
- GREENBERG L. J. Fluorometric measurement of alkaline phosphatase and aminopeptidase activities in the order of 10-14 mole. Biochem Biophys Res Commun. 1962 Nov 27;9:430–435. doi: 10.1016/0006-291x(62)90029-3. [DOI] [PubMed] [Google Scholar]
- Henis Y., Gould J. R., Alexander M. Detection and identification of bacteria by gas chromatography. Appl Microbiol. 1966 Jul;14(4):513–524. doi: 10.1128/am.14.4.513-524.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROTMAN B. Measurement of activity of single molecules of beta-D-galactosidase. Proc Natl Acad Sci U S A. 1961 Dec 15;47:1981–1991. doi: 10.1073/pnas.47.12.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]