Abstract
The cellular lipid patterns of seven strains of microorganisms were examined by gas-liquid chromatography in this preliminary study. The chloroform methanol-soluble lipids were extracted by the Soxhlet method from dried cultures which had been grown at 25 ± 2 C for 18 hr with mechanical shaking. The cellular extract was methylated by use of a low temperature sulfuric acid method, and the resulting methyl esters were chromatographed. Considerable differences in the lipid patterns among the seven microorganisms tested indicated that this method might be useful for the identification of closely related microbial genera, and possibly for species differentiation.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ABEL K., DESCHMERTZING H., PETERSON J. I. CLASSIFICATION OF MICROORGANISMS BY ANALYSIS OF CHEMICAL COMPOSITION. I. FEASIBILITY OF UTILIZING GAS CHROMATOGRAPHY. J Bacteriol. 1963 May;85:1039–1044. doi: 10.1128/jb.85.5.1039-1044.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bawdon R. E., Bassette R. Differentiation of Escherichia coli and Aerobacter aerogenes by gas liquid chromatography. J Dairy Sci. 1966 Jun;49(6):624–627. doi: 10.3168/jds.S0022-0302(66)87920-1. [DOI] [PubMed] [Google Scholar]
- Hagen P. O., Goldfine H., Williams P. J. Phospholipids of bacteria with extensive intracytoplasmic membranes. Science. 1966 Mar 25;151(3717):1543–1544. doi: 10.1126/science.151.3717.1543. [DOI] [PubMed] [Google Scholar]
- Henis Y., Gould J. R., Alexander M. Detection and identification of bacteria by gas chromatography. Appl Microbiol. 1966 Jul;14(4):513–524. doi: 10.1128/am.14.4.513-524.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MERDINGER E., DEVINE E. M., Jr LIPIDS OF DEBARYOMYCES HANSENII. J Bacteriol. 1965 Jun;89:1488–1493. doi: 10.1128/jb.89.6.1488-1493.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reiner E. Identification of bacterial strains by pyrolysis-gas-liquid chromatography. Nature. 1965 Jun 19;206(990):1272–1273. doi: 10.1038/2061272b0. [DOI] [PubMed] [Google Scholar]