Figure 3. Comparison between rIL-27 and rIL-15-mediated stimulation of resting NK cells.
NK cells purified from healthy donors were stimulated with rIL-15 (20 ng/ml) and rIL-27 (100 ng/ml) used alone or in combination. Cells were analyzed for (A) cytotoxicity against EA cells (51Cr release assay, E:T ratio 20:1), (B–G) expression of the indicated surface molecules (flow cytometry). Culture supernatants were analyzed for the presence of IFN-γ (ELISA assay) (H). Mean and 95% confidence intervals are indicated. *p < 0.05, p = ns means not significant. Data shown are pooled from 6 independent experiments performed using NK cells from 6 unrelated healthy donors.