Abstract
Tetrahymena pyriformis W with nutrients, ca. 22 × 106 cells, decreased the concentration of aflatoxin B1 58% in 24 hr and 67% in 48 hr. An unknown, bright-blue fluorescent substance was produced, with intensity about one-half that of the unchanged B1, with an Rf of 0.52 compared with 0.59 for B1 and 0.55 for B2 on a thin-layer chromatography plate, and with an ultraviolet spectrum showing maxima of 253, 261, and 328 mμ. In a separate assay, the cells with nutrients did not degrade pure G1. Starved, washed cells, ca. 11 × 106, decreased the concentration of B1 50% in 10 hr, 70% in 22 hr, and 75% in 30 hr, producing the same unknown component. Ethyl alcohol, 1.96% (v/v), decreased cell populations and size, but the cells remained actively motile in broth plus the alcohol for 96 hr. In 72 hr, neither toxin (ca. 2 ppm) in combination with ethyl alcohol had more inhibitory effect on cell numbers, with or without nutrients, than was produced by alcohol alone. Aflatoxin B1 had no observed effect on the viability of the starved cells for 30 hr or on the nourished cells for 72 hr. There was no noticeable effect of G1 on the starved cells in 30 hr or on the nourished cells in 48 hr. After 72 hr with G1 plus nutrients, many of the cells were round with blisters, nonmotile, and apparently dead or dying.
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