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. 1967 Sep;15(5):1160–1164. doi: 10.1128/am.15.5.1160-1164.1967

Copper Sulfate-induced Fermentation Changes in Continuous Cultures of the Rumen Microbial Ecosystem1

L L Slyter 1,2, M J Wolin 1
PMCID: PMC547158  PMID: 6077413

Abstract

The effect of CuSO4 on fermentation was studied in a continuously cultured rumen ecosystem. CuSO4, introduced at a level of 50 mg/500 ml of culture volume twice daily, caused a marked inhibition of fermentation of concentrates. Fermentation of alfalfa hay was not inhibited by the same CuSO4 concentration when the inoculum for the culture was obtained from a cow maintained on a normal concentrate ration. When the inoculum was from a cow on a high concentrate ration, hay fermentation was partially inhibited by CuSO4. Concentrations of CuSO4 that did not inhibit the fermentation of alfalfa hay or hay-concentrate mixtures caused preferential production of propionic acid and decreased production of methane.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Eden A., Green H. H. Micro-determination of copper in biological material. Biochem J. 1940 Sep;34(8-9):1202–1208. doi: 10.1042/bj0341202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. RUFENER W. H., Jr, NELSON W. O., WOLIN M. J. Maintenance of the rumen microbial population in continuous culture. Appl Microbiol. 1963 May;11:196–201. doi: 10.1128/am.11.3.196-201.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. SLYTER L. L., NELSON W. O., WOLIN M. J. MODIFICATIONS OF A DEVICE FOR MAINTENANCE OF THE RUMEN MICROBIAL POPULATION IN CONTINUOUS CULTURE. Appl Microbiol. 1964 Jul;12:374–377. doi: 10.1128/am.12.4.374-377.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Slyter L. L., Bryant M. P., Wolin M. J. Effect of pH on population and fermentation in a continuously cultured rumen ecosystem. Appl Microbiol. 1966 Jul;14(4):573–578. doi: 10.1128/am.14.4.573-578.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]

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