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. 1990 Sep;87(18):7230–7234. doi: 10.1073/pnas.87.18.7230

Characterization of three monoclonal antibodies that recognize the interferon alpha 2 receptor.

O R Colamonici 1, F D'Alessandro 1, M O Diaz 1, S A Gregory 1, L M Neckers 1, R Nordan 1
PMCID: PMC54717  PMID: 2144902

Abstract

The interferon system plays an important role in the control of viral infections and cell proliferation. These effects are mediated through the interaction of interferons with specific cell surface receptors. We report here the development of monoclonal antibodies against one of the subunits of the interferon alpha receptor. These antibodies detect a 110-kDa protein in surface-labeled cells and in Western blots, and 130- and 210-kDa bands after crosslinking to iodinated interferon alpha 2. No other subunits are disulfide-linked to the 130-kDa subunit or are coprecipitated by these antibodies. Analysis by two-dimensional gel electrophoresis revealed that the pI of this subunit is 3.5-5.0. We suggest that the protein recognized by these monoclonal antibodies be named the alpha subunit of the interferon alpha receptor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aguet M. High-affinity binding of 125I-labelled mouse interferon to a specific cell surface receptor. Nature. 1980 Apr 3;284(5755):459–461. doi: 10.1038/284459a0. [DOI] [PubMed] [Google Scholar]
  2. Billard C., Sigaux F., Castaigne S., Valensi F., Flandrin G., Degos L., Falcoff E., Aguet M. Treatment of hairy cell leukemia with recombinant alpha interferon: II. In vivo down-regulation of alpha interferon receptors on tumor cells. Blood. 1986 Mar;67(3):821–826. [PubMed] [Google Scholar]
  3. Branca A. A., Baglioni C. Evidence that types I and II interferons have different receptors. Nature. 1981 Dec 24;294(5843):768–770. doi: 10.1038/294768a0. [DOI] [PubMed] [Google Scholar]
  4. Clemens M. J., McNurlan M. A. Regulation of cell proliferation and differentiation by interferons. Biochem J. 1985 Mar 1;226(2):345–360. doi: 10.1042/bj2260345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Colamonici O. R., Ang S., Quinones R., Henkart P., Heikkila R., Gress R., Felix C., Kirsch I., Longo D., Marti G. IL-2-dependent expansion of CD3+ large granular lymphocytes expressing T cell receptor-gamma delta. Evidence for a functional receptor by anti-CD3 activation of cytolysis. J Immunol. 1988 Apr 15;140(8):2527–2533. [PubMed] [Google Scholar]
  6. Diaz M. O., Rubin C. M., Harden A., Ziemin S., Larson R. A., Le Beau M. M., Rowley J. D. Deletions of interferon genes in acute lymphoblastic leukemia. N Engl J Med. 1990 Jan 11;322(2):77–82. doi: 10.1056/NEJM199001113220202. [DOI] [PubMed] [Google Scholar]
  7. Diaz M. O., Ziemin S., Le Beau M. M., Pitha P., Smith S. D., Chilcote R. R., Rowley J. D. Homozygous deletion of the alpha- and beta 1-interferon genes in human leukemia and derived cell lines. Proc Natl Acad Sci U S A. 1988 Jul;85(14):5259–5263. doi: 10.1073/pnas.85.14.5259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Faltynek C. R., Branca A. A., McCandless S., Baglioni C. Characterization of an interferon receptor on human lymphoblastoid cells. Proc Natl Acad Sci U S A. 1983 Jun;80(11):3269–3273. doi: 10.1073/pnas.80.11.3269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gazdar A. F., Oie H. K., Kirsch I. R., Hollis G. F. Establishment and characterization of a human plasma cell myeloma culture having a rearranged cellular myc proto-oncogene. Blood. 1986 Jun;67(6):1542–1549. [PubMed] [Google Scholar]
  10. Golomb H. M. The treatment of hairy cell leukemia. Blood. 1987 Apr;69(4):979–983. [PubMed] [Google Scholar]
  11. Grossberg S. E., Taylor J. L., Kushnaryov V. M. Interferon receptors and their role in interferon action. Experientia. 1989 Jun 15;45(6):508–513. doi: 10.1007/BF01990499. [DOI] [PubMed] [Google Scholar]
  12. Hannigan G. E., Lau A. S., Williams B. R. Differential human interferon alpha receptor expression on proliferating and non-proliferating cells. Eur J Biochem. 1986 May 15;157(1):187–193. doi: 10.1111/j.1432-1033.1986.tb09655.x. [DOI] [PubMed] [Google Scholar]
  13. Joshi A. R., Sarkar F. H., Gupta S. L. Interferon receptors. Cross-linking of human leukocyte interferon alpha-2 to its receptor on human cells. J Biol Chem. 1982 Dec 10;257(23):13884–13887. [PubMed] [Google Scholar]
  14. Langer J. A., Pestka S. Interferon receptors. Immunol Today. 1988 Dec;9(12):393–400. doi: 10.1016/0167-5699(88)91241-8. [DOI] [PubMed] [Google Scholar]
  15. Miyakoshi J., Dobler K. D., Allalunis-Turner J., McKean J. D., Petruk K., Allen P. B., Aronyk K. N., Weir B., Huyser-Wierenga D., Fulton D. Absence of IFNA and IFNB genes from human malignant glioma cell lines and lack of correlation with cellular sensitivity to interferons. Cancer Res. 1990 Jan 15;50(2):278–283. [PubMed] [Google Scholar]
  16. Mogensen K. E., Bandu M. T., Vignaux F., Aguet M., Gressner I. Binding of 125I-labelled human alpha interferon to human lymphoid cells. Int J Cancer. 1981 Nov 15;28(5):575–582. doi: 10.1002/ijc.2910280508. [DOI] [PubMed] [Google Scholar]
  17. O'Farrell P. Z., Goodman H. M., O'Farrell P. H. High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell. 1977 Dec;12(4):1133–1141. doi: 10.1016/0092-8674(77)90176-3. [DOI] [PubMed] [Google Scholar]
  18. Pestka S., Langer J. A., Zoon K. C., Samuel C. E. Interferons and their actions. Annu Rev Biochem. 1987;56:727–777. doi: 10.1146/annurev.bi.56.070187.003455. [DOI] [PubMed] [Google Scholar]
  19. Quesada J. R., Gutterman J. U. Interferons in the treatment of human neoplasms. J Interferon Res. 1987 Oct;7(5):575–581. doi: 10.1089/jir.1987.7.575. [DOI] [PubMed] [Google Scholar]
  20. Quesada J. R., Reuben J., Manning J. T., Hersh E. M., Gutterman J. U. Alpha interferon for induction of remission in hairy-cell leukemia. N Engl J Med. 1984 Jan 5;310(1):15–18. doi: 10.1056/NEJM198401053100104. [DOI] [PubMed] [Google Scholar]
  21. Rubinstein M., Orchansky P. The interferon receptors. CRC Crit Rev Biochem. 1986;21(3):249–275. doi: 10.3109/10409238609113613. [DOI] [PubMed] [Google Scholar]
  22. Shibata T., Shimada Y., Shimoyama M. Time-dependent cytotoxic action of human recombinant alpha-interferon (Ro22-8181) in vitro and the sensitivity of various cultured leukemia and lymphoma cell lines to it. Jpn J Clin Oncol. 1985 Mar;15(1):67–75. [PubMed] [Google Scholar]
  23. Talpaz M., Kantarjian H. M., Kurzrock R., Gutterman J. Therapy of chronic myelogenous leukemia: chemotherapy and interferons. Semin Hematol. 1988 Jan;25(1):62–73. [PubMed] [Google Scholar]
  24. Talpaz M., Kantarjian H. M., McCredie K. B., Keating M. J., Trujillo J., Gutterman J. Clinical investigation of human alpha interferon in chronic myelogenous leukemia. Blood. 1987 May;69(5):1280–1288. [PubMed] [Google Scholar]
  25. Thompson M. R., Zhang Z., Fournier A., Tan Y. H. Characterization of human beta-interferon-binding sites on human cells. J Biol Chem. 1985 Jan 10;260(1):563–567. [PubMed] [Google Scholar]
  26. Uzé G., Lutfalla G., Gresser I. Genetic transfer of a functional human interferon alpha receptor into mouse cells: cloning and expression of its cDNA. Cell. 1990 Jan 26;60(2):225–234. doi: 10.1016/0092-8674(90)90738-z. [DOI] [PubMed] [Google Scholar]
  27. Zoon K. C., Arnheiter H. Studies of the interferon receptors. Pharmacol Ther. 1984;24(2):259–278. doi: 10.1016/0163-7258(84)90037-8. [DOI] [PubMed] [Google Scholar]

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