Abstract
Phenethyl β-d-galactopyranoside (PEG) was hydrolyzed by the β-galactosidase of Escherichia coli to form the toxic product phenethyl alcohol. Salmonella typhimurium did not hydrolyze PEG. In mixed culture, the ratio of S. typhimurium to E. coli was increased by growing the organisms in lactose broth containing 2.5% PEG. The high concentration of PEG required for inhibition of E. coli can be attributed to inadequate cell permeability rather than to prevention of β-galactosidase induction.
Full text
PDF





Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BERRAH G., KONETZKA W. A. Selective and reversible inhibition of the synthesis of bacterial deoxyribonucleic acid by phenethyl alcohol. J Bacteriol. 1962 Apr;83:738–744. doi: 10.1128/jb.83.4.738-744.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Butterfield C. T. The Selection of a Dilution Water for Bacteriological Examinations. J Bacteriol. 1932 May;23(5):355–368. doi: 10.1128/jb.23.5.355-368.1932. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fowler A. V., Zabin I. Effects of Dimethylsulfoxide on the Lactose Operon in Escherichia coli. J Bacteriol. 1966 Aug;92(2):353–357. doi: 10.1128/jb.92.2.353-357.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEDERBERG J. The beta-d-galactosidase of Escherichia coli, strain K-12. J Bacteriol. 1950 Oct;60(4):381–392. doi: 10.1128/jb.60.4.381-392.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEIVE L. A NONSPECIFIC INCREASE IN PERMEABILITY IN ESCHERICHIA COLI PRODUCED BY EDTA. Proc Natl Acad Sci U S A. 1965 Apr;53:745–750. doi: 10.1073/pnas.53.4.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MUELLER-HILL B., RICKENBERG H. V., WALLENFELS K. SPECIFICITY OF THE INDUCTION OF THE ENZYMES OF THE LAC OPERON IN ESCHERICHIA COLI. J Mol Biol. 1964 Nov;10:303–318. doi: 10.1016/s0022-2836(64)80049-8. [DOI] [PubMed] [Google Scholar]
- NORTH W. R., Jr Lactose pre-enrichment method for isolation of Samonella from dried egg albumen. Its use in a survery of commercially produced albumen. Appl Microbiol. 1961 May;9:188–195. doi: 10.1128/am.9.3.188-195.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pickett M. J., Goodman R. E. Beta-galactosidase for distinguishing between Citrobacter and Salmonella. Appl Microbiol. 1966 Mar;14(2):178–182. doi: 10.1128/am.14.2.178-182.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STOKES J. L., OSBORNE W. W. A selenite brilliant green medium for the isolation of Salmonella. Appl Microbiol. 1955 Jul;3(4):217–220. doi: 10.1128/am.3.4.217-220.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TAYLOR W. I., SILLIKER J. H., ANDREWS H. P. Isolation of Salmonellae from food samples. I. Factors affecting the choice of media for the detection and enumeration of Salmonella. Appl Microbiol. 1958 May;6(3):189–193. doi: 10.1128/am.6.3.189-193.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TAYLOR W. I., SILLIKER J. H. Isolation of Salmonellae from food samples. IV. Comparison of methods of enrichment. Appl Microbiol. 1961 Nov;9:484–486. doi: 10.1128/am.9.6.484-486.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
