Table 3.
Cross-reactions tested for Neospora caninum in published in-house ELISAs
| N. caninum ELISA | Parasitic species for which cross-reactivity was examined | Reference |
|---|---|---|
| Whole tachyzoite lysate antigen ELISA (various extraction protocols) | T. gondii | (Pare et al. 1995a; Dubey et al. 1996) |
| Hammondia spp. | (Dubey et al. 1996) | |
| Sarcocystis. spp. | (Pare et al. 1995a; Dubey et al. 1996)a | |
| Eimeria spp. | (Pare et al. 1995a; Dubey et al. 1996) | |
| Cryptosporidium spp. | (Pare et al. 1995a; Dubey et al. 1996) | |
| T. gondii, Sarcocystis spp., Babesia spp. | (Osawa et al. 1998) | |
| T. gondii, Sarcocystis spp., Eimeria spp., Babesia spp., Cryptosporidium spp. | (Wouda et al. 1998) | |
| T. gondii | (Silva et al. 2007) | |
| Fixed whole tachyzoites ELISA | T. gondii | (Williams et al. 1997) |
| Sarcocystis spp. | (Williams et al. 1997)a | |
| Eimeria spp. | (Williams et al. 1997) | |
| Babesia spp. | (Williams et al. 1997)a | |
| Cryptosporidium spp. | (Williams et al. 1997) | |
| ISCOM incorporated antigen, indirect | T. gondii | (Björkman et al. 1994)b, (Björkman et al. 1997), (Björkman and Hemphill, 1998)c, (Björkman and Lunden, 1998)d, |
| Sarcocystis spp. | (Björkman et al. 1994)b, (Björkman et al. 1997) | |
| Eimeria spp., Babesia spp. | (Björkman et al. 1997) | |
| Native NcSRS2, indirect | T. gondii | (Schares et al. 2000; Hosseininejad et al. 2010) |
| Sarcocystis spp., Eimeria spp., Babesia spp., Cryptosporidium spp. | (Schares et al. 2000) | |
| Leishmania infantum | (Hosseininejad et al. 2010) | |
| Antigen-capture indirect (polyclonal antiserum) | T. gondii, Sarcocystis spp., Eimeria spp., Babesia spp., Cryptosporidium spp. | (Schares et al. 1999b) |
| Antigen-capture competitive ELISA (Mabs 5B6-25 and 4A4-2) | T. gondii, Sarcocystis spp. | (Baszler et al. 2001) |
| Competitive ELISA (4A4-2) | T. gondii | (Baszler et al. 1996)e |
| Sarcocystis spp. | (Baszler et al. 1996)e | |
| Competitive ELISA (rabbit polyclonal antibody) | T. gondii, Sarcocystis spp., Eimeria spp., Babesia spp. | (McGarry et al. 2000) |
a
Cross-reactions observed in serum dilutions lower than the cutoff.
b
Rabbit sera against T. gondii and S. cruzi do not react in WB with ISCOM antigen.
c
MAbs against ISCOM antigens do not recognize T. gondii in WB.
d
Mouse serum immunized with ISCOM antigen does not recognize T. gondii.
e
Some sera showed elevated levels of cross-reactions in WB.