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. 1967 Nov;15(6):1270–1283. doi: 10.1128/am.15.6.1270-1283.1967

Production of Lysergic Acid Derivatives in Submerged Culture

IV. Inorganic Nutrition Studies with Claviceps paspali

John P Rosazza 1, William J Kelleher 1, Arthur E Schwarting 1
PMCID: PMC547183  PMID: 16349735

Abstract

The inorganic requirements for growth and alkaloid production by submerged cultures of a lysergic acid alkaloid-producing isolate of Claviceps paspali were determined in a defined medium. The requirement for peak growth was essentially the same as the requirement for maximal alkaloid production in the case of potassium and magnesium. With phosphorus, however, maximal growth was reached at lower levels than those required for maximal alkaloid production. Sulfur was required in smaller amounts for peak alkaloid production than for maximal growth. In the case of iron and zinc, the requirement for maximal alkaloid production was greater than for growth. Manganese and copper could be sufficiently depleted to demonstrate their essentiality for alkaloid production, but their requirements for growth could not be demonstrated.

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Selected References

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