Abstract
Some of the physiological and biochemical characteristics of a type F strain recently isolated from the United States were studied and compared with those of the prototype Langeland type F strain. The recent isolates were nonproteolytic, fermented sucrose and ribose, produced spores of low thermal resistance, produced a protoxin activated by trypsin, and grew and produced toxin at 38 F (3.3 C) from a spore inoculum. The prototype Langeland strain was proteolytic, did not ferment sucrose or ribose, and produced spores of relatively high thermal resistance, and the toxin of 3-day-old cultures was not activated by trypsin. Approximately two to three times the minimal lethal dose (MLD) of type F toxin from either Langeland or nonproteolytic strains was cross-neutralized by 1,000 anti-MLD of type E antitoxin. Antitoxin serums prepared by immunizing rabbits with the toxoid of the nonproteolytic type F isolate neutralized the toxin of the Langeland strain, but did not show cross-neutralization with the toxins of other types of Clostridium botulinum.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Craig J. M., Pilcher K. S. Clostridium botulinum Type F: Isolation from Salmon from the Columbia River. Science. 1966 Jul 15;153(3733):311–312. doi: 10.1126/science.153.3733.311. [DOI] [PubMed] [Google Scholar]
- DOLMAN C. E. Additional botulism episodes in Canada. Can Med Assoc J. 1954 Sep;71(3):245–249. [PMC free article] [PubMed] [Google Scholar]
- DOLMAN C. E. Recent observations on type E botulism. Can J Public Health. 1957 May;48(5):187–198. [PubMed] [Google Scholar]
- DUFF J. T., WRIGHT G. G., YARINSKY A. Activation of Clostridium botulinum type E toxin by trypsin. J Bacteriol. 1956 Oct;72(4):455–460. doi: 10.1128/jb.72.4.455-460.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eklund M. W., Wieler D. I., Poysky F. T. Outgrowth and toxin production of nonproteolytic type B Clostridium botulinum at 3.3 to 5.6 C. J Bacteriol. 1967 Apr;93(4):1461–1462. doi: 10.1128/jb.93.4.1461-1462.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holdeman L. V., Smith L. D. Study of the nutritional requirements and toxin production of Clostridium botulinum type F. Can J Microbiol. 1965 Dec;11(6):1009–1019. doi: 10.1139/m65-134. [DOI] [PubMed] [Google Scholar]
- LAYTON W. M., Jr, HALLESY D. W. DEFORMITY OF FORELIMB IN RATS: ASSOCIATION WITH HIGH DOSES OF ACETAZOLAMIDE. Science. 1965 Jul 16;149(3681):306–308. doi: 10.1126/science.149.3681.306-a. [DOI] [PubMed] [Google Scholar]
- McClung L. S., Toabe R. The Egg Yolk Plate Reaction for the Presumptive Diagnosis of Clostridium sporogenes and Certain Species of the Gangrene and Botulinum Groups. J Bacteriol. 1947 Feb;53(2):139–147. doi: 10.1128/jb.53.2.139-147.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WILLIS A. T., HOBBS G. A medium for the identification of clostridia producing opalescence in egg-yolk emulsions. J Pathol Bacteriol. 1958 Apr;75(2):299–305. doi: 10.1002/path.1700750208. [DOI] [PubMed] [Google Scholar]
- WILLIS A. T., HOBBS G. Some new media for the isolation and identification of Clostridia. J Pathol Bacteriol. 1959 Apr;77(2):511–521. doi: 10.1002/path.1700770223. [DOI] [PubMed] [Google Scholar]
- Wentz M. W., Scott R. A., Vennes J. W. Clostridium botulinum type F: seasonal inhibition by bacillus licheniformis. Science. 1967 Jan 6;155(3758):89–90. doi: 10.1126/science.155.3758.89. [DOI] [PubMed] [Google Scholar]