Table 2.
BG1-Env-8ANC195 complex cryo-EM structure data collection and model statistics.
| Data collection/processing | |
|---|---|
| Microscope | Titan krios |
| Voltage | 300 kV |
| Camera | Gatan K2 Summit |
| Camera mode | counting |
| Defocus range | 1.5–3.5 |
| Exposure time | 15 s |
| Dosage rate | 2.92 electrons⋅pixel−1⋅subframe−1 |
| Magnified pixel size | 1.35 |
| Total Dose (e/A2) | 80 |
| Reconstruction | |
| Software | Relion-1.4 |
| Symmetry | C1 |
| Particles refined | |
| Resolution(automask, Å) | 6.18 |
| Resolution(masked out Fab CHCL domains, Å) | 6.18 |
| Map sharpening B-factor (Å2) | −24.7274 |
| Model Statistics | |
| Map CC (whole unit cell): | 0.931 |
| Map CC (around atoms): | 0.731 |
| All-atom clashscore | 17.66 |
| Ramachandran plot: | |
| outliers: | 0.44% |
| allowed: | 7.44% |
| favored: | 92.12% |
| rmsd (bonds): | 0.01 |
| rmsd (angles): | 1.11 |
| Rotamer outliers: | 0.85% |
| C-beta deviations: | 0 |